ADIPOSE ATROPHY OF EXPCRINE PANCREAS
ADIPOSE ATROPHY OF EXPCRINE PANCREAS
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DOI：
10.1002/path.1700920232
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发表时间：
1966-01-01
期刊：
影响因子：
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通讯作者：
WALTERS, MNI
中科院分区：
文献类型：
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作者：
WALTERS, MNI
材料与方法从200例几乎连续的成人尸检中获得胰腺头部、体部和尾部的组织块;在4%中性甲醛中固定后，用石蜡浸渍组织，并按以下方法对切片进行染色：Harris苏木精、天青石蓝和伊红、picro-Mallory和过碘酸-希夫（PAS）技术。在选定的病例中，用Gordon和Sweets的方法浸渍网硬蛋白（Culling，1963）。冰冻切片油红0染色。组织中的脂肪量从0到4任意分级：1级和2级代表头、体或尾实质中少于或多于8个脂肪陷窝; 3级表示外分泌小叶的部分脂肪替代区域（图1），4级表示全部替代区域（图2）。
MATERIALS AND METHODS Blocks of the head, body and tail of the pancreas were obtained from 200 almost consecutive necropsies on adults; after fixation in 4 per cent. neutral formaldehyde the tissues were impregnated with paraffin wax and sections stained as follows: Harris's haematoxylin, celestin blue and eosin, the picro-Mallory and periodic acid-Schiff (PAS) techniques. In selected cases reticulin was impregnated by the method of Gordon and Sweets (Culling, 1963). Frozen sections were stained with oil red 0.Grading of fatty change. The amount of fat in the tissues was graded arbitrarily from 0 to 4: grades 1 and 2 represent less or more than 8 fatty lacunes in the parenchyma of the head, body or tail; grade 3 indicates areas of partial fatty replacement of exocrine lobules (fig. l), and grade 4 represents areas of total replacement (fig. 2).