Automated flow cytometric analysis of cerebrospinal fluid.
Automated flow cytometric analysis of cerebrospinal fluid.
复制标题
脑脊液的自动流式细胞术分析。
DOI：
10.1093/clinchem/47.3.556
复制
发表时间：
2001
影响因子：
9.3
通讯作者：
Alain Verstraete
中科院分区：
文献类型：
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作者：
Jos T. Van Acker;J. Delanghe;M. Langlois;Y. Taes;M. D. Buyzere;Alain Verstraete
背景
最近，UF-100（Sysmex Corporation）流式细胞仪被开发用于自动化尿液分析。我们评估了流式细胞术在脑脊液（CSF）分析中的应用。
方法
UF-100数据与256份CSF样本的显微镜检查和生化数据相关。对144例脑膜炎疑似病例进行了微生物分析。
结果
UF-100和显微镜数据之间获得了良好的一致性，红细胞（r = 0.919）和白细胞（r = 0.886）。然而，在某些情况下，UF-100对淋巴细胞的不正确分类导致白细胞计数被低估。UF-100细菌计数与UF-100白细胞计数（r = 0.666）、CSF总蛋白（r = 0.754）、CSF乳酸浓度（r = 0.641）呈正相关（P < 0.001），与CSF葡萄糖浓度呈负相关（r = -0.405; P < 0.001）。UF-100细菌计数在出血样本和脑室引流收集的样本中不可靠，其中观察到血小板和细胞碎片的干扰。另一个主要问题是UF-100在无菌CSF样本中的“细菌”背景信号。用流式细胞仪检测颅咽管瘤中新生隐球菌、酵母细胞和胆固醇结晶。
结论
使用UF-100的CSF流式细胞术可提供快速可靠的白细胞和红细胞计数。仪器提供的其他设置可能有助于诊断神经系统疾病。
BACKGROUND
Recently, the UF-100 (Sysmex Corporation) flow cytometer was developed to automate urinalysis. We evaluated the use of flow cytometry in the analysis of cerebrospinal fluid (CSF).
METHODS
UF-100 data were correlated with microscopy and biochemical data for 256 CSF samples. Microbiological analysis was performed in 144 suspected cases of meningitis.
RESULTS
Good agreement was obtained between UF-100 and microscopy data for erythrocytes (r = 0.919) and leukocytes (r = 0.886). In some cases, however, incorrect classification of lymphocytes by the UF-100 led to underestimation of the leukocyte count. UF-100 bacterial count positively correlated (P < 0.001) with UF-100 leukocyte count (r = 0.666), CSF total protein (r = 0.754), and CSF lactate concentrations (r = 0.641), and negatively correlated with CSF glucose concentration (r = -0.405; P < 0.001). UF-100 bacterial counts were unreliable in hemorrhagic samples and in samples collected by ventricular drainage where interference by blood platelets and cell debris was observed. Another major problem was the UF-100 "bacterial" background signal in sterile CSF samples. Cryptococcus neoformans yeast cells and cholesterol crystals in craniopharyngioma were detected by the flow cytometer.
CONCLUSIONS
Flow cytometry of CSF with the UF-100 offers a rapid and reliable leukocytes and erythrocyte count. Additional settings offered by the instrument may be useful in the diagnosis of neurological disorders.