Artificial photosynthetic cell producing energy for protein synthesis
Artificial photosynthetic cell producing energy for protein synthesis
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DOI：
10.1038/s41467-019-09147-4
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发表时间：
2019-03-22
影响因子：
16.6
通讯作者：
Kuruma, Yutetsu
中科院分区：
文献类型：
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作者：
Berhanu, Samuel;Ueda, Takuya;Kuruma, Yutetsu
构建人造细胞的尝试拓宽了我们对生物体的理解。许多细胞内系统已经通过组装分子来重建，然而据我们所知，通过自给自足的能量合成其自身成分的机制尚未开发出来。在这里，我们将无细胞蛋白质合成系统和由纯化的 ATP 合酶和细菌视紫红质组成的小蛋白脂质体结合在巨大的单层囊泡内，通过光产生 ATP 来合成蛋白质。光合成的 ATP 作为转录底物和翻译能量被消耗，最终驱动细菌视紫红质或 ATP 合酶组成蛋白（蛋白脂质体的原始重要成分）的合成。从头进行光合作用的细菌视紫红质和部分ATP合酶整合到人工光合细胞器中，通过产物的正反馈增强其ATP光合活性。我们的人工光合细胞系统为构建能量独立的人工细胞铺平了道路。
Attempts to construct an artificial cell have widened our understanding of living organisms. Many intracellular systems have been reconstructed by assembling molecules, however the mechanism to synthesize its own constituents by self-sufficient energy has to the best of our knowledge not been developed. Here, we combine a cell-free protein synthesis system and small proteoliposomes, which consist of purified ATP synthase and bacteriorhodopsin, inside a giant unilamellar vesicle to synthesize protein by the production of ATP by light. The photo-synthesized ATP is consumed as a substrate for transcription and as an energy for translation, eventually driving the synthesis of bacteriorhodopsin or constituent proteins of ATP synthase, the original essential components of the proteoliposome. The de novo photosynthesized bacteriorhodopsin and the parts of ATP synthase integrate into the artificial photosynthetic organelle and enhance its ATP photosynthetic activity through the positive feedback of the products. Our artificial photosynthetic cell system paves the way to construct an energetically independent artificial cell.