Characteristics of photodissociation at 193 nm of singly protonated peptides generated by matrix-assisted laser desorption ionization (MALDI)
Characteristics of photodissociation at 193 nm of singly protonated peptides generated by matrix-assisted laser desorption ionization (MALDI)
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DOI：
10.1016/j.jasms.2006.07.021
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发表时间：
2006-12-01
影响因子：
3.2
通讯作者：
Kim, Myung Soo
中科院分区：
文献类型：
--
作者：
Choi, Kyung Mi;Yoon, So Hee;Kim, Myung Soo
研究了各种单质子化多肽在193 nm处的光解离。这些包括在C-末端、N-末端、在两个末端、在链内具有精氨酸残基的肽，以及没有精氨酸残基的肽。对前体离子进行单同位素选择。通过使用偏转系统（先前报告）并从激光开启信号中减去剩余信号，尽可能减少来自源后衰减（PSD）产物信号的干扰。如前所述，发现精氨酸残基及其在肽内的位置的存在显著影响PD光谱。脯氨酸、天冬氨酸或谷氨酸残基的存在几乎不影响PD光谱模式。通过比较在几个不同波长下获得的PD谱，得出结论：光激发离子的解离发生在其基态电子态。还提出了基于反应的统计图的所观察到的光谱相关性的尝试性解释。
Photodissociation (PD) at 193 nm of various singly protonated peptides Was investigated. These include peptides with an arginine residue at the C-terminus, N-terminus, at both termini, inside the chain, and those without an arginine residue. Monoisotopomeric selection was made for the precursor ions. Interference from the post-source decay (PSD) product signals was reduced as much as possible by using the deflection system (reported previously) and subtracting the remaining signals from the laser-on signals. The presence of an arginine residue and its position inside the peptide were found to significantly affect the PD spectra, as reported previously. Presence of a proline, aspartic acid, or glutamic acid residue hardly affected the PD spectral patterns. By comparing the PD spectra obtained at a few different wavelengths, it is concluded that the dissociation of the photoexcited ions occurs in their ground electronic states. Tentative explanations for the observed spectral correlations based on the statistical picture for the reactions are also presented.