Comparison of in vitro Serum Protein Binding of Thyroxin and Triiodothyronine.∗
Comparison of in vitro Serum Protein Binding of Thyroxin and Triiodothyronine.∗
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甲状腺素和三碘甲状腺原氨酸的体外血清蛋白结合比较。*
DOI：
10.3181/00379727-84-20696
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发表时间：
1953
期刊：
影响因子：
--
通讯作者：
F. Larson
中科院分区：
文献类型：
--
作者：
W. P. Deiss;E. Albright;F. Larson
摘要1.在体外实验上的血清蛋白结合甲状腺素和三碘甲状腺原氨酸进行纸电泳分离的蛋白质组分和1131标记的三碘甲状腺原氨酸和甲状腺素，以跟踪这些氨基酸的定位。2.虽然甲状腺素和三碘甲状腺原氨酸均被α球蛋白结合，但甲状腺素结合更牢固，不易被三碘甲状腺原氨酸取代，而三碘甲状腺原氨酸结合不牢固，容易被甲状腺素取代。3.被发现位移三碘甲状腺原氨酸跟踪甲状腺素结合球蛋白，而被认为是与甲状腺素置换白蛋白馏分。4.这些观察结果可能与静脉注射后三碘甲腺原氨酸的快速消失和血液中三碘甲腺原氨酸水平相对较低有关。
Summary 1. In vitro experiments on the serum protein binding of thyroxin and triiodothyronine were performed using paper electrophoresis to separate the protein fractions and 1131 labelled triiodothyronine and thyroxin to trace the localization of these amino acids. 2. Although both thyroxin and triiodothyronine were shown to be bound by alpha globulin, thyroxin was more firmly bound and not easily displaced by triiodothyronine whereas triiodothyronine was less firmly bound and readily displaced by thyroxin. 3. Displaced triiodothyronine was found to trail the thyroxin-binding globulin, whereas displaced thyroxin was seen to associate with the albumin fraction. 4. These observations are possibly related to the rapid disappearance of triiodothyronine after intravenous injection and to the relatively low levels of triiodothyronine in blood.