Repair of a Rev-minus human immunodeficiency virus type 1 mutant by activation of a cryptic splice site.
Repair of a Rev-minus human immunodeficiency virus type 1 mutant by activation of a cryptic splice site.
复制标题
通过激活隐秘剪接位点修复 Rev-minus 人类免疫缺陷病毒 1 型突变体。
DOI：
10.1128/jvi.75.7.3495-3500.2001
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发表时间：
2001
影响因子：
5.4
通讯作者：
Berkhout,B
中科院分区：
文献类型：
--
作者：
Verhoef,K;Bilodeau,PS;vanWamel,JL;Kjems,J;Stoltzfus,CM;Berkhout,B
We isolated a revertant virus after prolonged culturing of a replication-impaired human immunodeficiency virus type 1 (HIV-1) mutant of which the Rev open reading frame was inactivated by mutation of the AUG translation initiation codon. Sequencing of thetat-revregion of this revertant virus identified a second-site mutation intatthat restored virus replication in the mutant background. This mutation activated a cryptic 5′ splice site (ss) that, when used in conjunction with the regular HIV 3′ ss #5, fuses thetatandrevreading frames to encode a novel T-Rev fusion protein that rescues Rev function. We also demonstrate an alternative route to indirectly activate this cryptic 5′ ss by mutational inactivation of an adjacent exon splicing silencer element.
