Optical transillumination tomography for imaging of tissue-engineered blood vessels
Optical transillumination tomography for imaging of tissue-engineered blood vessels
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DOI：
10.1007/s10439-005-1734-x
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发表时间：
2005-03-01
影响因子：
3.8
通讯作者：
Haidekker, MA
中科院分区：
文献类型：
--
作者：
Gladish, JC;Yao, G;Haidekker, MA
组织工程的最新进展导致从患者自身细胞中生长出完全生物化的人体血管。这些组织工程血管 (TEBV) 是单独生长的，每个项目的成本很高，因此需要密切的生长监测和质量控制。我们设计并测试了一种光学透射断层扫描系统，使用红色激光对弱散射样本（例如 TEBV）进行成像。对固定的 TEBV 进行成像，并将结果与光学相干断层扫描进行比较。该初步扫描仪原型的面内分辨率为 50 μm，可以看到样品中微小的不均匀性和缺陷。发现组织衰减为70 cm(-1)。与光学相干断层扫描相比，透射断层扫描仪的主要优点是仪器便宜，并且可以使用 CCD 相机快速获取完整的 3D 切片。本研究中提出的原型为进一步提高图像质量和采集速度提供了基础。
Recent progress in tissue engineering led to the development of completely biological human vessels grown from the patient's own cells. Those tissue-engineered blood vessels (TEBV) are grown on an individual basis at high costs per item, and therefore require close growth monitoring and quality control. We designed and tested an optical transillumination tomography system using red laser light to image weakly scattering specimens, such as TEBV. Fixated TEBV were imaged and the results compared to optical coherence tomography. This preliminary scanner prototype had an in-plane resolution of 50 mu m and allowed to see small inhomogeneities and defects in the samples. Tissue attenuation was found to be 70 cm(-1). Main advantages of the transillumination tomography scanner over optical coherence tomography were the inexpensive instrumentation and the potential to rapidly acquire complete 3D sections with a CCD camera. The prototype presented in this study provides a basis to further improve image quality and acquisition speed.