REGULATION OF A SEGMENTATION STRIPE BY OVERLAPPING ACTIVATORS AND REPRESSORS IN THE DROSOPHILA EMBRYO
REGULATION OF A SEGMENTATION STRIPE BY OVERLAPPING ACTIVATORS AND REPRESSORS IN THE DROSOPHILA EMBRYO
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DOI：
10.1126/science.1683715
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发表时间：
1991-11-29
期刊：
影响因子：
56.9
通讯作者：
LEVINE, M
中科院分区：
文献类型：
--
作者：
STANOJEVIC, D;SMALL, S;LEVINE, M
果蝇胚胎中的基因表达条纹为真核启动子如何响应转录调控因子的组合而打开和关闭提供了一个模型。遗传学研究表明，偶数跳条纹2是由三个缺口基因，驼背（hb），Kruppel（Kr），和巨人（gt），并由母体形态原bicoid（bcd）控制。在条纹2启动子元件中的这些调节蛋白的结合位点与早期胚胎发生期间条纹的表达之间建立了直接联系。bcd和hb蛋白结合位点介导激活，而相邻的gt和Kr蛋白位点抑制表达并建立条纹边界。条纹2具有遗传开关的特性。
Gene expression stripes in Drosophila melanogaster embryos provide a model for how eukaryotic promoters are turned on and off in response to combinations of transcriptional regulators. Genetic studies suggested that even-skipped (eve) stripe 2 is controlled by three gap genes, hunchback (hb), Kruppel (Kr), and giant (gt), and by the maternal morphogen bicoid (bcd). A direct link is established between binding sites for these regulatory proteins in the stripe 2 promoter element and the expression of the stripe during early embryogenesis. The bcd and hb protein binding sites mediate activation, whereas neighboring gt and Kr protein sites repress expression and establish the stripe borders. The stripe 2 clement has the properties of a genetic on-off switch.