Scanning electron microscopic studies of bullfrog sympathetic neurons exposed by enzymatic removal of connective tissue elements and satellite cells
Scanning electron microscopic studies of bullfrog sympathetic neurons exposed by enzymatic removal of connective tissue elements and satellite cells
复制标题
通过酶法去除结缔组织成分和卫星细胞而暴露的牛蛙交感神经元的扫描电子显微镜研究
DOI：
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
P. Baluk
中科院分区：
文献类型：
--
作者：
P. Baluk
对牛蛙的第九和第十腹交感神经节去除神经元上的结缔组织成分后进行了光镜、透射和扫描电子显微镜观察。用胰酶消化组织和随后的酸解暴露了单极神经元，这些神经元仍然被它们的卫星细胞覆盖。节前神经支配见于节后突起的近段和轴突丘区。神经节周围可见的小细胞群可能与富含儿茶酚胺颗粒的细胞群(SIF细胞)相对应。胶原酶和蛋白水解酶消化除去了结缔组织以外的部分或全部卫星细胞。真正的神经元表面有短的指状突起，而卫星细胞的外表面光滑。节前神经曲张清晰可见于节后神经突起近端、轴索丘和神经元胞体。少数轴索静脉曲张破裂，露出突触内的小泡。讨论了静脉曲张的分布和神经胶质包膜对其功能的可能影响。
SummaryThe ninth and tenth abdominal sympathetic ganglia of bullfrogs were studied by light microscopy and transmission and scanning electron microscopy after the removal of the connective tissue elements overlying the neurons. Digestion of tissues with trypsin and subsequent acid hydrolysis exposed the unipolar neurons, which remained covered by their satellite cells. The preganglionic innervation was visible on the proximal segment and axon hillock region of the postganglionic neurite. Clusters of small cells seen at the periphery of ganglia probably corresponded to groups of cells with abundant catecholamine-containing granules (SIF cells). Digestion with collagenase and protease removed some or all of the satellite cells in addition to the connective tissue. The true neuronal surfaces had short finger-like processes, whereas the external surfaces of satellite cells were smooth. Preganglionic nerve varicosities were clearly visible on the proximal segment of the postganglionic neurite, on the axon hillock and on the cell body of neurons. A few axonal varicosities were fractured to reveal the synaptic vesicles within. The possible effects of the distribution and glial ensheathment of nerve varicosities on their function are discussed.
神经释放的递质快速激活突触前烟碱乙酰胆碱受体。
DOI：
10.1111/j.1460-9568.2003.03064.x
发表时间：
2003
期刊：
The European journal of neuroscience
影响因子：
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作者：
Rogers,Marc;Sargent,PeterB
通讯作者：
Sargent,PeterB