Glycolysis preferentially inhibits ATP-sensitive K+ channels in isolated guinea pig cardiac myocytes.
Glycolysis preferentially inhibits ATP-sensitive K+ channels in isolated guinea pig cardiac myocytes.
复制标题
DOI：
10.1126/science.2443972
复制
发表时间：
1987-10
期刊：
影响因子：
56.9
通讯作者：
J. Weiss;S. Lamp
中科院分区：
文献类型：
--
作者：
J. Weiss;S. Lamp
在心脏方面，糖酵解可能是膜功能所需的三磷酸腺苷(ATP)的优先来源。本研究采用膜片钳技术研究了通透性心肌细胞钾通道对细胞内ATP水平的敏感性。这些K+通道的激活与细胞内显著的K+丢失有关，从而导致心肌缺血时的电生理异常和心律失常。结果表明，糖酵解比氧化磷酸化能更有效地阻止ATP敏感性钾通道的开放。在切除的由内向外的斑块中的实验表明，位于细胞膜或邻近通道的细胞骨架中的关键糖酵解酶可能解释了他们对糖酵解ATP的偏好。
In heart, glycolysis may be a preferential source of adenosine triphosphate (ATP) for membrane functions. In this study the patch-clamp technique was used to study potassium channels sensitive to intracellular ATP levels in permeabilized ventricular myocytes. Activation of these K+ channels has been implicated in marked cellular K+ loss leading to electrophysiological abnormalities and arrhythmias during myocardial ischemia. The results showed that glycolysis was more effective than oxidative phosphorylation in preventing ATP-sensitive K+ channels from opening. Experiments in excised inside-out patches suggested that key glycolytic enzymes located in the membrane or adjacent cytoskeleton near the channels may account for their preference for glycolytic ATP.