Isolated P2 rRNA promoters of Escherichia coli are strong promoters that are subject to stringent control.
Isolated P2 rRNA promoters of Escherichia coli are strong promoters that are subject to stringent control.
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DOI：
10.1006/jmbi.1994.1641
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发表时间：
1994-10
影响因子：
5.6
通讯作者：
R. Gafny;S. Cohen;N. Nachaliel;G. Glaser
中科院分区：
文献类型：
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作者：
R. Gafny;S. Cohen;N. Nachaliel;G. Glaser
大肠杆菌中核糖体RNA的合成受到严格控制。这七个rRNA操纵子是高度保守的，每个都是从两个串联启动子P1和P2转录的，它们相距约120个碱基对。在指数生长的细胞中，大多数转录物起始于P1启动子。P1启动子受到高度调节，并且受到严格的生长速度控制。在这里，我们表明，从rrnA P1启动子转录减少P2的表达。在P1不存在的情况下，P2启动子充当相当强的启动子。在P1和P2之间插入一个转录终止子消除了P1对P2的抑制，这表明RNA聚合酶的物理运动起源于P1并沿着P2启动子进行是干扰过程发生所必需的。与P1相似，P2启动子也受到严格控制。
Ribosomal RNA synthesis in Escherichia coli is under stringent control. The seven rRNA operons are highly conserved and are each transcribed from two tandem promoters, P1 and P2, which are located about 120 base-pairs apart. In exponentially growing cells the majority of the transcripts are initiated at the P1 promoters. The P1 promoters are highly regulated, and are under stringent as well as growth rate controls. Here we demonstrate that transcription from the rrnA P1 promoter diminishes P2 expression. In the absence of P1, the P2 promoter acts as a rather strong promoter. Insertion of a transcription terminator between P1 and P2 eliminates the inhibition of P2 by P1, suggesting that the physical movement of RNA polymerase originating at P1 and progressing along the P2 promoter is necessary for the interference process to take place. Similarly to P1, the solitary P2 promoter is subject to stringent control.