Transgenerational activation of an autonomous DNA transposon, Dart1-24, by 5-azaC treatment in rice
Transgenerational activation of an autonomous DNA transposon, Dart1-24, by 5-azaC treatment in rice
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通过 5-azaC 处理水稻，自主 DNA 转座子 Dart1-24 的跨代激活
DOI：
10.1007/s00122-019-03429-7
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发表时间：
2019
影响因子：
5.4
通讯作者：
Maekawa M.
中科院分区：
文献类型：
--
作者：
2.Nishimura H;Himi E;Eun CH;Takahashi H;Qian Q;*Tsugane K;Maekawa M.
关键信息edart1 -24是37个自主DNA转座子dart1之一，在5- azac处理水稻种子后，通过5 '区域的去甲基化被遗传激活。转座子受表观遗传调控。为了获得水稻DNA转座子dar1的新激活的自主元件，用5-azaC（一种去甲基化剂）处理了一个稳定的淡黄色叶片（pyl-stb）突变体的种子，该突变体是由osclpp5中的非自主元件ndart1 -0插入引起的。在5-azaC处理的M1植株中，60-70%的植株表现出淡黄色叶片（pyl-v）表型，这取决于所使用的5-azaC浓度，这表明失活的达特1可能在5-azaC处理下被高度激活，并且达特1-0被活化的达特1s从mosclpp5中切除。虽然由这些pyl-v植株衍生的M2植株表现出稳定的pyl表型，但一些M1杂交种产生了pyl-v的M2后代。这些结果表明，M1上的大多数M1 pyl-v表型是不可遗传的。在M2 pyl-v植株中表达了Dart1-24、1-27和1-28，定位分析证实了Dart1-24是新激活的。此外，达特1-24的跨代激活被证明是由其5 '区核苷酸的去甲基化引起的。
Key messageDart1-24, one of the 37 autonomous DNA transposonDart1s, was heritably activated by the demethylation of the 5′ region following 5-azaC treatment of rice seeds.AbstractTransposons are controlled by epigenetic regulations. To obtain newly activated autonomous elements ofDart1, a DNA transposon, in rice, seeds of a stable pale yellow leaf (pyl-stb) mutant caused by the insertion ofnDart1-0, a nonautonomous element inOsClpP5,were treated with 5-azaC, a demethylating agent. In the 5-azaC-treated M1 plants, 60–70% of the plants displayed variegated pale yellow leaf (pyl-v) phenotype, depending on the concentration of 5-azaC used, suggesting that inactivatedDart1might become highly activated by 5-azaC treatment andnDart1-0was excised fromOsClpP5by the activatedDart1s. Although the M2 plants derived from most of these pyl-v plants showed stable pyl phenotypes, some variegated M1 plants generated pyl-v M2 progeny. These results indicated that most M1 pyl-v phenotypes at M1 were not heritable.Dart1-24,1-27and1-28were expressed in the M2 pyl-v plants, and mapping analysis confirmed thatDart1-24was newly activated. Further, the transgenerational activation ofDart1-24was demonstrated to be caused by the demethylation of nucleotides in its 5′ region.