Osteoblastic networks with deficient coupling: differential effects of magnetic and electric field exposure.
Osteoblastic networks with deficient coupling: differential effects of magnetic and electric field exposure.
复制标题
耦合不足的成骨细胞网络：磁场和电场暴露的差异效应。
DOI：
10.1016/s8756-3282(00)00315-x
复制
发表时间：
2000
期刊：
影响因子：
4.1
通讯作者：
McLeod,KJ
中科院分区：
文献类型：
--
作者：
VanderMolen,MA;Donahue,HJ;Rubin,CT;McLeod,KJ
利用间隙连接缺陷的细胞系来测试细胞间耦合是否在调节生物物理刺激（例如细胞外电流）的影响中发挥重要作用。将骨肉瘤细胞系 ROS 17/2.8 细胞以及对照转染细胞系和连接蛋白 43 间隙连接缺陷细胞系暴露于时变磁通量（30 Hz，1.8 毫特斯拉），足以在培养物中诱导 2 mV/m 量级的电场。场暴露抑制细胞生长，与间隙连接耦合无关，而碱性磷酸酶活性被发现依赖于间隙连接耦合。这些发现可以解释为表明磁场和电场暴露对细胞培养物具有不同的影响，磁场暴露通过独立于间隙连接耦合的机制抑制细胞生长，而酶活性的改变似乎是通过间隙连接依赖性方式受到感应电场的刺激。
A gap junction-deficient cell line was utilized to test whether intercellular coupling plays a significant role in modulating the influence of biophysical stimuli such as extracellular electrical currents. ROS 17/2.8 cells, an osteosarcoma cell line, along with a control transfected cell line and a connexin 43-gap junction-deficient cell line, were exposed to a time-changing magnetic flux (30 Hz, 1.8 milliTesla) sufficient to induce an electric field in the cultures on the order of 2 mV/m. Field exposure inhibited cell growth independent of gap junctional coupling, while alkaline phosphatase activity was found to be dependent on gap junctional coupling. These findings can be interpreted to suggest that magnetic and electric field exposures have differential effects on cell cultures, with magnetic field exposure inhibiting cell growth through a mechanism independent of gap junctional coupling, while the alteration in enzyme activity appears to be stimulated by the induced electric field in a gap junction-dependent manner.