Screening for tick-borne and tick-associated viruses in ticks collected in Ghana
Screening for tick-borne and tick-associated viruses in ticks collected in Ghana
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DOI：
10.1007/s00705-021-05296-4
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发表时间：
2021-11-10
影响因子：
2.7
通讯作者：
Isawa, Haruhiko
中科院分区：
文献类型：
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作者：
Amoa-Bosompem, Michael;Kobayashi, Daisuke;Isawa, Haruhiko
蜱是吸血的节肢动物，传播许多病原体，包括虫媒病毒。由蜱传播的虫媒病毒通常被称为蜱传病毒（tick-borne viruses，TBV）。已知TBV会引起人类、宠物和牲畜的疾病。然而，关于撒哈拉以南非洲地区结核病毒的发生和分布情况的资料非常有限。这项研究旨在确定加纳犬和牛感染蜱的存在和分布，以及确定蜱传播或蜱相关病毒。一个更多样化的人口蜱被发现感染牛（三个属）相对于那些感染狗（一个属）。在通过RT-PCR筛选的蜱池中检测到六种白蛉病毒和一种正鼻病毒。随后的序列分析揭示了两种不同的静脉病毒和先前报道的从狗收集的蜱中的Odaw病毒，以及一种与从牛收集的蜱中的四种未分类静脉病毒最密切相关的病毒（16 GH-T27）。病毒16 GH-T27被认为是Balambala蜱病毒（BTV）的一种毒株，并命名为BTV毒株16 GH-T27。BTV阳性蜱虫池的下一代测序分析仅检测到L和S片段。系统发育分析表明，BTV与以前定义为M节段缺陷型静脉病毒的病毒聚类。从牛身上采集的蜱中检出的正鼻病毒经确认为具有重要医学意义的杜格贝病毒。此外，我们还讨论了了解蜱和TBV的存在和分布在疾病预防和缓解中的重要性以及对公共卫生的影响。我们的研究结果有助于TBV和蜱相关病毒的知识库。
Ticks are blood-sucking arthropods that transmit many pathogens, including arboviruses. Arboviruses transmitted by ticks are generally referred to as tick-borne viruses (TBVs). TBVs are known to cause diseases in humans, pets, and livestock. There is, however, very limited information on the occurrence and distribution of TBVs in sub-Saharan Africa. This study was designed to determine the presence and distribution of ticks infesting dogs and cattle in Ghana, as well as to identify the tick-borne or tick-associated viruses they harbour. A more diverse population of ticks was found to infest cattle (three genera) relative to those infesting dogs (one genus). Six phleboviruses and an orthonairovirus were detected in tick pools screened by RT-PCR. Subsequent sequence analysis revealed two distinct phleboviruses and the previously reported Odaw virus in ticks collected from dogs and a virus (16GH-T27) most closely related to four unclassified phleboviruses in ticks collected from cattle. The virus 16GH-T27 was considered a strain of Balambala tick virus (BTV) and named BTV strain 16GH-T27. Next-generation sequencing analysis of the BTV-positive tick pool detected only the L and S segments. Phylogenetic analysis revealed that BTV clustered with viruses previously defined as M-segment-deficient phleboviruses. The orthonairovirus detected in ticks collected from cattle was confirmed to be the medically important Dugbe virus. Furthermore, we discuss the importance of understanding the presence and distribution of ticks and TBVs in disease prevention and mitigation and the implications for public health. Our findings contribute to the knowledge pool on TBVs and tick-associated viruses.