Structure-function relationships and mode of replication of animal rhabdoviruses.
Structure-function relationships and mode of replication of animal rhabdoviruses.
复制标题
动物弹状病毒的结构-功能关系和复制模式。
DOI：
10.1073/pnas.72.3.933
复制
发表时间：
1975
影响因子：
11.1
通讯作者：
H. Koprowski
中科院分区：
文献类型：
--
作者：
F. Sokol;H. Koprowski
最近积累的知识允许更精确地比较几种不同的动物弹状病毒的结构（和可能的进化）关系：水泡性口炎病毒，狂犬病病毒，克恩峡谷病毒，鲤鱼病毒的春季病毒血症。每种病毒主要由糖蛋白、RNA相关核蛋白和一种或两种膜蛋白组成。水泡性口炎病毒组病毒含有较少量的另外两种不同的多肽，NS和L。分离的病毒根据特征模式在体内经历结构多肽磷酸化。在水泡性口炎病毒中，NS蛋白被选择性磷酸化。在狂犬病组病毒和鲤鱼病毒的春季病毒血症中，核蛋白是主要的磷蛋白;在这些病毒中，只有磷酸化部分被胰蛋白酶选择性地切除。在克恩峡谷病毒中，只有膜蛋白和糖蛋白被弱磷酸化。每种病毒都有一个病毒体结合的蛋白激酶。水泡性口炎病毒组病毒、克恩峡谷病毒和鲤鱼春季病毒血症病毒只含有病毒体结合的转录酶，其活性水平在体外分别降低。水泡性口炎病毒和克恩峡谷病毒在去核细胞中有效复制，狂犬病病毒则不能。根据这些观察，它建议，水泡性口炎病毒可能代表最高度进化的这些弹状病毒，而春季病毒血症的鲤鱼和克恩峡谷病毒可能代表水泡性口炎和狂犬病病毒组之间的“进化联系”。
Recently accumulated knowledge allows more precise comparison of the structural (and possibly evolutionary) relationships of several different animal rhabdoviruses: vesicular stomatitis virus, rabies virus, Kern Canyon virus, and spring viremia of carp virus. Each virus is composed primarily of a glycoprotein, an RNA-associated nucleoprotein, and one or two membrane proteins. Vesicular stomatitis virus group viruses contain lesser amounts of two additional distinct polypeptides, NS and L. The separate viruses undergo structural polypeptide phosphorylation in vivo according to characteristic patterns. In vesicular stomatitis virus the NS protein is selectively phosphorylated. In rabies group viruses and in spring viremia of carp virus, the nucleoprotein is the predominant phosphoprotein; in these viruses only the phosphorylated moiety is selectively cleaved off with trypsin. In Kern Canyon virus, only membrane protein and glycoprotein are weakly phosphorylated. Each virus possesses a virion-bound protein kinase. Vesicular stomatitis virus group viruses, Kern Canyon virus, and spring viremia of carp virus only contain virion-bound transcriptases of respectively decreasing levels of activity demonstrable in vitro. Vesicular stomatitis and Kern Canyon viruses replicate efficiently in enucleated cells; rabies virus does not. Based upon these observations, it is suggested that vesicular stomatitis virus may represent the most highly evolved of these rhabdoviruses, whereas spring viremia of carp and Kern Canyon viruses may represent "evolutionary links" between the vesicular stomatitis and rabies virus groups.