Suppression of splenic accessory cell function in mice exposed to gallium arsenide.
Suppression of splenic accessory cell function in mice exposed to gallium arsenide.
复制标题
暴露于砷化镓的小鼠脾脏辅助细胞功能的抑制。
DOI：
10.1016/0041-008x(91)90297-r
复制
发表时间：
1991
影响因子：
3.8
通讯作者：
Munson,AE
中科院分区：
文献类型：
--
作者：
Sikorski,EE;Burns,LA;McCoy,KL;Stern,M;Munson,AE
小鼠急性暴露于单一腹腔内剂量的砷化镓（50、100和200 mg/kg）可通过改变脾辅助细胞的功能抑制对T依赖性抗原绵羊红细胞（SRBC）的一级IgM抗体反应。为了确定GaAs暴露影响脾辅助细胞的机制，通过粘附分离细胞，并在动物中GaAs暴露后24小时研究其功能能力。GaAs暴露小鼠的脾贴壁细胞在其处理和呈递颗粒抗原SRBC至SRBC引发的T细胞群体的能力方面大大受损。然而，GaAs暴露并没有抑制这些细胞的吞噬荧光covaspheres，也没有抑制在体内吞噬51铬标记SRBC，表明在这里报道的结果是不是由于减少抗原的辅助细胞的摄取。此外，暴露小鼠的这些细胞产生的IL-1与对照组没有什么不同，并且向培养物中添加外源性IL-1并不能逆转GaAs诱导的对一抗反应的抑制。GaAs暴露不影响Ia阳性巨噬细胞（F4 80阳性细胞）的百分比，但通过流式细胞术测量，细胞表面IAK分子表达的量显著降低。与SRBC反应相反，GaAs没有抑制粘附脾细胞处理和呈递抗原鸽子细胞色素c的能力，以辅助/诱导T细胞克隆F1.A.2或抗原KLH（钥孔血蓝蛋白）KLH-引发的T细胞。因此，GaAs暴露干扰脾巨噬细胞处理和/或呈递颗粒抗原SRBC的能力，但不干扰可溶性蛋白抗原鸽子细胞色素c或KLH。
Acute exposure of mice to a single intratracheal dose of gallium arsenide (50, 100, and 200 mg/kg) depresses the primary IgM antibody response to the T-dependent antigen sheep red blood cells (SRBC) through alterations in the function of splenic accessory cells. To determine the mechanism by which GaAs exposure influences splenic accessory cells, the cells were isolated by adherence and their functional capability investigated 24 hr following GaAs exposure in the animal. Splenic adherent cells from GaAs-exposed mice were greatly impaired in their ability to process and present the particulate antigen SRBC to a SRBC-primed T-cell population. However, GaAs exposure did not inhibit phagocytosis of fluorescent covaspheres by these cells, nor did it inhibit in vivo phagocytosis of51Cr-labeled SRBC, indicating that the findings reported here were not due to decreased uptake of antigen by the accessory cells. Furthermore, production of Il-1 by these cells from exposed mice was not different from control and addition of exogenous IL-1 to cultures did not reverse GaAs-induced inhibition of the primary antibody response. GaAs exposure did not affect the percentage of Ia positive macrophages (F4 80 positive cells), but the amount of cell surface IAkmolecules expressed was significantly decreased as measured by flow cytometry. In contrast to the SRBC response, GaAs did not suppress the ability of adherent splenocytes to process and present the antigen pigeon cytochrome c to the helper/inducer T cell clone F1.A.2 or the antigen KLH (keyhole limpet hemocyanin) to KLH-primed T cells. Therefore, GaAs exposure interferes with the capacity of splenic macrophages to process and/or present the particulate antigen SRBC, but not the soluble protein antigens pigeon cytochrome c or KLH.