Electrical stimulation of a denervated muscle promotes selective reinnervation by native over foreign motoneurons
Electrical stimulation of a denervated muscle promotes selective reinnervation by native over foreign motoneurons
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DOI：
10.1152/jn.00451.2001
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发表时间：
2002-04-01
影响因子：
2.5
通讯作者：
Garren, KC
中科院分区：
文献类型：
--
作者：
Zealear, DL;Rodriguez, RJ;Garren, KC
本实验观察了电刺激失神经支配的环鳗后肌(PCA)对其再支配的影响。8只动物被植入一个由36个电极组成的平面阵列，用于慢性刺激和记录肌肉对整个扇形表面的自发和诱发肌电(EMG)电位。记录单侧神经切断前各电极部位的标准肌电数据，神经切断后记录受累伙伴的标准肌电数据。将动物随机分为实验组和对照组，切断并吻合支配Pca外展肌及其内收肌拮抗剂的右侧喉返神经。在11个月的实验中，连续刺激4只实验动物的前列腺癌肌肉，使用1-S，30-pps的双相脉冲序列，由1-ms脉冲组成，幅度2-6 mA，每10个S重复一次。其余4只动物作为非刺激对照组。自然吸气运动神经元的适当再支配通过声带张开的大小和所有电极部位的电位来指示行为。通过记录刺激喉粘膜感觉神经传入反射性诱发的EMG电位，对外来内收肌运动神经元的不适当再支配进行定量。与对照组相比，所有四只实验动物都显示出更高水平的正确的前列腺癌肌肉神经再支配(P<；0.0064)和更低水平的错误神经再支配(P<；0.0084)。直接肌肉刺激似乎也增强了神经再支配的总体幅度，但效果没有那么强(P<；0.113)。这些发现与之前的一份报告一致，表明刺激哺乳动物肌肉可能会深刻地影响其对特定运动神经元类型再神经支配的接受性。
The effect of electrical stimulation of the denervated posterior cricoarytenoid (PCA) muscle on its subsequent reinnervation was explored in the canine. Eight animals were implanted with a planar array of 36 electrodes for chronic stimulation and recording of spontaneous and evoked electromyographic (EMG) potentials across the entire fan-shaped surface of a muscle pair. Normative EMG data were recorded from each electrode site before unilateral nerve section, and from the innervated partner after nerve section. After randomizing the animals to experimental and control groups, the right recurrent laryngeal nerve innervating the PCA abductor muscle and its adductor antagonists was sectioned and reanastomosed. The PCA muscle in four experimental animals was continuously stimulated during the 11-mo experiment, using a 1-s, 30-pps, biphasic pulse train composed of 1-ms pulses 2-6 mA in amplitude and repeated every 10 s. The remaining four animals served as nonstimulated controls. Appropriate reinnervation by native inspiratory motoneurons was indexed behaviorally by the magnitude of vocal fold opening and electromyographically by the potential across all electrode sites. Inappropriate reinnervation by foreign adductor motoneurons was quantitated by recording EMG potentials evoked reflexly by stimulation of sensory afferents of the laryngeal mucosa. All four experimental animals showed a greater level of correct PCA muscle reinnervation (P < 0.0064) and a lesser level of incorrect reinnervation (P < 0.0084) than the controls. Direct muscle stimulation also appeared to enhance the overall magnitude of reinnervation, but the effect was not as strong (P < 0.113). These findings are consistent with a previous report and suggest that stimulation of a mammalian muscle may profoundly affect its receptivity to reinnervation by a particular motoneuron type.