Induction of Female-to-Male Sex Reversal by High Temperature Treatment in Medaka, Oryzias latipes
Induction of Female-to-Male Sex Reversal by High Temperature Treatment in Medaka, Oryzias latipes
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DOI：
10.2108/zsj.22.985
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发表时间：
2005-09
影响因子：
4.1
通讯作者：
Tadashi Sato;T. Endo;K. Yamahira;S. Hamaguchi;M. Sakaizumi
中科院分区：
文献类型：
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作者：
Tadashi Sato;T. Endo;K. Yamahira;S. Hamaguchi;M. Sakaizumi
青鳉（Oryzias latipes）具有稳定的XX-XY性别决定系统，Y染色体上含有性别决定基因DMY。然而，先前的研究表明，高水温可能会影响青鳉的性别决定。本研究探讨了高水温对性反转的影响。两种青鳉近交系的受精卵在高水温（32°C）下发育直至孵化。将孵化的鱼苗保持在正常水温（27°C）下直到成年，并检查表型和基因型性别。结果，24%（N=105）和50%（N=36）的XX鱼在Hd-rR和HNI近交系中分别形成雄性表型。这些XX性别反转的雄性动物具有正常的睾丸，并且具有完全的生育能力。另一方面，所有XY鱼在两个品系中均为雄性。这些结果表明，高水温可以诱导XX性逆转，并在胚胎阶段的水温升高是一个简单而有用的方法来获得XX男性青鳉。
Abstract Medaka, Oryzias latipes, has a firm XX-XY sex-determining system with the sex-determining gene, DMY, on the Y chromosome. However, previous studies have suggested that high water temperature might affect sex determination in Medaka. In the present study, the influence of high water temperature on sex reversal was examined. Fertilized eggs of two inbred strains of Medaka were developed at high water temperature (32°C) until hatching. The hatched fry were kept at normal water temperatures (27°C) until adulthood, and the phenotypic and genotypic sex was examined. As a result, 24% (N=105) and 50% (N=36) of XX fish developed a male phenotype in the Hd-rR and HNI inbred strains, respectively. These XX sex-reversed males had a normal testis and were fully fertile. On the other hand, all XY fish were male in the both strains. These results demonstrate that high water temperatures can induce XX sex reversal and that elevated water temperatures during the embryonic stage is a simple and useful method for getting XX males in Medaka.