A molecular analysis of prokaryotic and viral DNA sequences in prostate tissue from patients with prostate cancer indicates the presence of multiple and diverse microorganisms
A molecular analysis of prokaryotic and viral DNA sequences in prostate tissue from patients with prostate cancer indicates the presence of multiple and diverse microorganisms
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DOI：
10.1002/pros.20680
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发表时间：
2008-02-15
期刊：
影响因子：
2.8
通讯作者：
Isaacs, William B.
中科院分区：
文献类型：
--
作者：
Sfanos, Karen S.;Sauvageot, Jurga;Isaacs, William B.
背景。急性和慢性炎症是前列腺组织学的共同特征。虽然炎症被认为在前列腺良性和恶性生长中发挥重要作用，但这种炎症的刺激因素仍然知之甚少。传染性病原体是前列腺炎症的潜在刺激因素。方法。使用通用真细菌 PCR 检测了 30 名癌症患者的 170 份前列腺组织核心样本的 16S rDNA 基因序列。对阳性 PCR 产物 (n = 64, 37%) 进行克隆和测序。为了进行比较，使用标准临床微生物技术培养了 30 名患者的组织样本。通过有机体特异性 PCR 检测了另外 200 名患者的 DNA 样本中是否存在沙眼衣原体、痤疮丙酸杆菌、阴道毛滴虫、BK 病毒、EB 病毒、人巨细胞病毒、人乳头瘤病毒和异种鼠白血病相关病毒。 结果。 16S 测序结果表明存在 83 种不同的微生物。微生物培养分离出的物种明显较少。一般来说，生物体特异性 PCR 无法检测到先前报道的前列腺中常见的多种生物体。特定细菌种类的存在与急性或慢性炎症的组织学证据之间没有显着关联。结论。大多数接受前列腺切除术的男性 (87%) 的前列腺含有来自一种或多种物种的细菌 DNA。然而，大多数个体组织核心样本呈阴性，表明细菌存在的区域异质性以及缺乏普遍或普遍存在的前列腺菌群。培养结果表明，前列腺中存在的物种具有“不可培养”的性质，或者 16S rDNA 序列源自非活细菌。
BACKGROUND. Inflammation, both acute and chronic, is a common feature of Prostate histology. While inflammation has been proposed to play an important role in both benign and malignant growth of the prostate, the stimuli for this inflammation remain poorly characterized. Infectious pathogens are potential stimuli for prostatic inflammation.METHODS. Universal eubacterial PCR was used to test 170 prostate tissue core samples from 30 cancer patients for 16S rDNA gene sequences. Positive PCR products (n = 64, 37%) were cloned and sequenced. For comparison, tissue samples from 30 patients were cultured using standard clinical microbiological techniques. DNA samples from 200 additional patients were tested by organism-specific PCR for the presence of Chlamydia trachomatis, Propionibacterium acnes, Trichomonas vaginalis, BK virus, Epstein-Barr virus, human cytomegalovirus, human papillomavirus, and xenotropic murine leukemia-related virus.RESULTS. 16S sequencing results indicated the presence of 83 distinct microorganisms. Microbiological culture isolated markedly fewer species. In general, organism-specific PCR failed to detect multiple organisms previously reported as common in the prostate. There was no significant association between the presence of particular species of bacteria and histologic evidence of acute or chronic inflammation.CONCLUSIONS. Most prostates from men undergoing prostatectomy (87%) contain bacterial DNA from one or more species. However, the majority of individual tissue core samples were negative, suggesting regional heterogeneity in the presence of bacteria and a lack of a generalized or ubiquitous prostatic flora. Culture results suggest either the "unculturable" nature of species present in the prostate or that 16S rDNA sequences were derived from nonviable bacteria.