Wolbachia manipulates reproduction of spider mites by influencing herbivore salivary proteins
Wolbachia manipulates reproduction of spider mites by influencing herbivore salivary proteins
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DOI：
10.1002/ps.7201
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发表时间：
2022-10-05
影响因子：
4.1
通讯作者：
Hong, Xiao-Yue
中科院分区：
文献类型：
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作者：
Bing, Xiao-Li;Xia, Cai-Bei;Hong, Xiao-Yue
研究背景内共生沃尔巴克氏菌以控制宿主繁殖而著称。沃尔巴克氏菌还可以通过调节植物和草食动物之间的相互作用来影响宿主的适合性。然而，目前尚不清楚唾液蛋白是否参与了这一过程。结果发现沃尔巴克氏菌感染可使危害1000种植物的叶螨Tetranychus urticae Koch的产卵量减少，孵化率增加。转录学和蛋白质组学分析表明，Wolbachia感染的蠕形螨上调了许多鞭毛虫唾液蛋白的基因表达水平，其中包括一组叶蝉科特有的、功能不具特征的SHOT1s(叶蝉科分泌的寄主反应蛋白)。SHOT1基因在叶螨的取食期(若虫和成虫)中的表达高于在卵中的表达，并在原毛虫中高度集中。RNA干扰实验表明，SHOT1基因的敲除显著降低了沃尔巴克氏菌的密度，增加了产卵量，降低了卵的孵化率。结论SHOT1与沃尔巴克氏菌密度呈正相关，可解释沃尔巴克氏菌介导的表型。我们的结果提供了新的证据，表明食草动物唾液蛋白与沃尔巴克氏菌介导的对植物寄主行为的操纵有关。(C)2022年化学工业学会。
BACKGROUND The endosymbiont Wolbachia is known for manipulating host reproduction. Wolbachia also can affect host fitness by mediating interactions between plant and herbivores. However, it remains unclear whether saliva proteins are involved in this process. RESULTS We found that Wolbachia infection decreased the number of deposited eggs but increased the egg hatching rate in the spider mite Tetranychus urticae Koch (Acari: Tetranychidae), a cosmopolitan pest that infects >1000 species of plants. Transcriptomic and proteomic analyses revealed that Wolbachia-infected mites upregulated the gene expression levels of many T. urticae salivary proteins including a cluster of Tetranychidae-specific, functionally uncharacterized SHOT1s (secreted host-responsive proteins of Tetranychidae). The SHOT1 genes were expressed more in the feeding stages (nymphs and adults) of mites than in eggs and highly enriched in the proterosomas. RNA interference experiments showed that knockdown of SHOT1s significantly decreased Wolbachia density, increased the number of deposited eggs and decreased the egg hatching rate. CONCLUSION Together, these results indicate that SHOT1s are positively correlated with Wolbachia density and account for Wolbachia-mediated phenotypes. Our results provide new evidence that herbivore salivary proteins are related to Wolbachia-mediated manipulations of host performance on plants. (c) 2022 Society of Chemical Industry.