MALAT-1: A long non-coding RNA and its important 3′ end functional motif in colorectal cancer metastasis
MALAT-1: A long non-coding RNA and its important 3′ end functional motif in colorectal cancer metastasis
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DOI：
10.3892/ijo.2011.1007
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发表时间：
2011-07-01
影响因子：
5.2
通讯作者：
Li, Zuguo
中科院分区：
文献类型：
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作者：
Xu, Chuan;Yang, Minhui;Li, Zuguo
人类转移相关肺腺癌转录物 1 (MALAT-1) 是一种长非编码 RNA，已知在许多被检测出患有癌症的人中被错误调节。我们早期的研究发现MALAT-1在结直肠癌（CRC）转移中发挥着关键作用。在这项研究中，我们分析了 MALAT-1 基因的五个片段。我们采用测序过程来识别以下类型样本中的 MALAT-1 突变：CRC 细胞（SW620、SW480）、正常结直肠组织和原发性 CRC 组织。我们成功检测到以下突变：MALAT-1的5434 nt-6951 nt片段在SW620细胞中发生突变，而MALAT-1的5434 nt-6951 nt和6918 nt-8441 nt片段在SW480癌细胞和原发性CRC组织中发生突变。我们在 CRC 细胞系 SW480 中过表达 MALAT-1 的 5 个片段；同时确保MALAT-1低表达 我们的数据表明，位于MALAT-1 3端的5个片段之一（6918 nt-8441 nt）在细胞增殖、迁移和侵袭的生物学过程中发挥着关键作用。基于这些观察，我们可以推断MALAT-1的3'端是CRC细胞侵袭和转移的重要生物基序。我们成功地提出了第一个证据，表明在 CRC 中的长非编码 RNA MALAT-1 上发现了突变。此外，长非编码RNA MALAT-1在CRC中具有位于MALAT-1 (6918 nt-8441 nt) 3'端的重要生物基序。我们的研究为主要致力于探索结直肠癌侵袭和转移过程中发生的分子机制的研究提供了新的方向。
The human metastasis associated lung adeno carcinoma transcript 1 (MALAT-1) as a long non-coding RNA known to be misregulated in many people who are detected with cancer. Our earlier studies found that MALAT-1 plays a pivotal role in colorectal cancer (CRC) metastasis. In this study, we analyzed the MALAT-1 gene in five fragments. We employed the sequencing process to identify MALAT-1 mutations in the following types of samples: CRC cells (SW620, SW480), normal colorectal tissues, and primary CRC tissues. We were successful in detecting the following mutations: fragment 5434 nt-6951 nt of the MALAT-1 was mutated in SW620 cells, while fragments 5434 nt-6951 nt and 6918 nt-8441 nt of MALAT-1 were mutated in SW480 cancer cells and primary CRC tissues. We over-expressed five fragments of MALAT-1 in the CRC cell line SW480; simultaneously ensuring that MALAT-1 had low expression Our data illustrated that one of the 5 fragments (6918 nt-8441 nt) located at the 3 end of MALAT-1 plays a pivotal role in the biological processes of cell proliferation, migration and invasion. Based on these observations, we can infer that the 3' end of MALAT-1 is an important biological motif in the invasion and metastasis of CRC cells. We have successfully presented the first evidence that mutations were found on the long non-coding RNA MALAT-1 in CRC. Moreover, long non-coding RNA MALAT-1 has an important biological motif located at the 3' end of MALAT-1 (6918 nt-8441 nt) in CRC. Our study gives a new direction to research primarily focused on exploring the molecular mechanisms occurring during the invasion and metastasis of CRC.