Enzyme-linked immunosorbent assay to detect urinary antibody against recombinant rKRP42 antigen made from Leishmania donovani for the diagnosis of visceral leishmaniasis
Enzyme-linked immunosorbent assay to detect urinary antibody against recombinant rKRP42 antigen made from Leishmania donovani for the diagnosis of visceral leishmaniasis
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DOI：
10.4269/ajtmh.2008.79.599
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发表时间：
2008-10-01
影响因子：
3.3
通讯作者：
Kimura, Eisaku
中科院分区：
文献类型：
--
作者：
Islam, Mohammad Zahidul;Itoh, Makoto;Kimura, Eisaku
我们最近报道了分子量为 42 kd 的杜氏利什曼原虫驱动蛋白相关重组蛋白 (rKRP42) 的生产，以及该抗原在基于血清 ELISA 中诊断内脏利什曼病 (VL) 的价值。在本研究中，使用尿液样本（rKRP42 尿液 ELISA）通过 ELISA 验证了 rKRP42 抗原。基于尿液的 ELISA 显示出 94% 的敏感性（115 个 VL 样本中的 108 个阳性）和 99.6% 的特异性（240 个非 VL 样本中的 239 个阴性）。灵敏度和特异性与我们之前使用丙酮处理的杜氏乳杆菌前鞭毛体抗原进行 ELISA 和直接凝集试验的结果几乎相似，这两种方法都是使用尿液样本进行的。使用 108 个 VL 样本将 rKRP42 尿液 ELISA 与市售尿液抗原检测试剂盒 (KAtex) 进行比较，结果显示 ELISA 的灵敏度 (96.3%) 远高于 KAtex (55.6%)。在尿液样本中使用 rKRP42 抗原将有助于流行病学研究。
We recently reported the production of the recombinant kinesin-related protein of Leishmania donovani with a molecular weight of 42 kd (rKRP42) and the value of the antigen in serum-based ELISA for the diagnosis of visceral leishmaniasis (VL). In this study, the rKRP42 antigen was validated with ELISA using urine samples (rKRP42 urine ELISA). The urine-based ELISA showed 94% sensitivity (108 positives among 115 VL samples) and 99.6% specificity (239 negatives among 240 non-VL samples). The sensitivity and specificity are almost similar to our previous results by ELISA with acetone-treated L. donovani promastigote antigen and direct agglutination test, both methods being done by use of urine samples. A comparison of the rKRP42 urine ELISA with the commercially available urinary antigen detection kit (KAtex) using 108 VL samples showed much higher sensitivity of the ELISA (96.3%) than KAtex (55.6%). The use of the rKRP42 antigen with urine samples will facilitate epidemiologic studies.