One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides
One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides
复制标题
一锅合成壳聚糖包覆磁性胶体纳米晶簇选择性富集糖肽
DOI：
10.1016/j.aca.2014.05.037
复制
发表时间：
2014-09-02
影响因子：
6.2
通讯作者：
Zou, Hanfa
中科院分区：
文献类型：
--
作者：
Fang, Chunli;Xiong, Zhichao;Zou, Hanfa
由于非糖基化肽的共存引起电离期间的离子抑制效应，在质谱（MS）分析之前选择性富集糖肽是必不可少的。在这些富集方法中，基于磁分离的亲水作用液相色谱（HILIC）是近年来比较流行的方法。由于这些材料的常规合成方法繁琐且耗时，至少需要四步。本文采用简单的一锅法成功制备了壳聚糖包覆的磁性胶体Fe3O4@CS MCCs。所得Fe3O4@CS MCCs表现出优异的糖肽富集能力，具有高选择性、低检测限和高结合容量。在对真实的复杂生物样品的分析中，对45 μ g HeLa细胞蛋白样品进行3次重复分析，共鉴定出283个独特的N-糖基化位点，对应于175个糖基化蛋白，表明在糖蛋白组学分析中对低丰度糖肽的检测和鉴定具有很大的潜力。(C)2014爱思唯尔有限公司版权所有。
Selective enrichment of glycopeptides prior to the mass spectrometry (MS) analysis is essential due to ion suppression effect during ionization caused by the co-presence of non-glycosylated peptides. Among the enrichment approaches, hydrophilic interaction liquid chromatography (HILIC) based on magnetic separation has become a popular method in recent years. As the conventional synthesis procedures of these materials are tedious and time-consuming with at least four steps. Herein, magnetic colloidal nanocrystal clusters coated with chitosan (Fe3O4@CS MCNCs) have been successfully prepared by a simple one-pot method. The resulting Fe3O4@CS MCNCs demonstrated an excellent ability for glycopeptide enrichment with high selectivity, low detection limit and high binding capacity. Furthermore, in the analysis of real complicated biological sample, 283 unique N-glycosylation sites corresponding to 175 glycosylated proteins were identified in three replicate analyses of 45 mu g protein sample extracted from HeLa cells, indicating the great potential in detection and identification of low abundant glycopeptides in glycoproteome analysis. (C) 2014 Elsevier B.V. All rights reserved.