Iron-related transcriptomic variations in CaCo-2 cells, an in vitro model of intestinal absorptive cells
Iron-related transcriptomic variations in CaCo-2 cells, an in vitro model of intestinal absorptive cells
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DOI：
10.1152/physiolgenomics.00297.2005
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发表时间：
2006-06-16
影响因子：
4.6
通讯作者：
Mosser, Jean
中科院分区：
文献类型：
--
作者：
Chicault, Celine;Toutain, Bertrand;Mosser, Jean
调节十二指肠肠细胞对铁的吸收是通过防止铁缺乏或过载来维持体内平衡所必需的。尽管已经确定了一些与铁吸收及其调节有关的基因，但很可能还有更多的因素有待确定。为此，我们使用了全局转录组学方法，使用CaCo-2细胞系作为肠道吸收细胞的体外模型。泛基因组筛选与细胞内铁含量相关的基因表达变异使我们鉴定出171个基因。这些基因中的109个被聚集成5种类型的表达谱。这是第一次发现这些基因中的大多数与铁代谢有关。这五个簇的功能注释表明免疫反应，蛋白质水解过程和铁消耗之间的潜在联系。相反，铁过载与细胞代谢有关，尤其是脂质和谷胱甘肽的代谢，涉及氧化还原功能和电子转移。
Regulation of iron absorption by duodenal enterocytes is essential for the maintenance of homeostasis by preventing iron deficiency or overload. Despite the identification of a number of genes implicated in iron absorption and its regulation, it is likely that further factors remain to be identified. For that purpose, we used a global transcriptomic approach, using the CaCo-2 cell line as an in vitro model of intestinal absorptive cells. Pangenomic screening for variations in gene expression correlating with intracellular iron content allowed us to identify 171 genes. One hundred nine of these genes are clustered into five types of expression profile. This is the first time that most of these genes have been associated with iron metabolism. Functional annotation of these five clusters indicates potential links between the immune response, proteolysis processes, and iron depletion. In contrast, iron overload is associated with cellular metabolism, especially that of lipids and glutathione involving redox function and electron transfer.