Distribution of particulate matter and tissue remodeling in the human lung.
Distribution of particulate matter and tissue remodeling in the human lung.
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DOI：
10.1289/ehp.001081063
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发表时间：
2000-11
影响因子：
10.4
通讯作者：
Schenker MB
中科院分区：
文献类型：
--
作者：
Pinkerton KE;Green FH;Saiki C;Vallyathan V;Plopper CG;Gopal V;Hung D;Bahne EB;Lin SS;Ménache MG;Schenker MB
我们研究了来自加州中央谷的西班牙裔男性肺内碳质和矿物质粉尘颗粒分布与气道沿着解剖学上不同气道路径重塑之间的关系。弗雷斯诺县验尸官办公室的肺尸检标本通过在30 cm H（2）O压力下气管内滴注2%戊二醛制备。两个不同的气道路径进入左上肺叶的尖后和尖前部分。用于组织学分析的组织样本通常取自肺内第二、第四、第六和第九代气道。还分析了每个气道路径的第12代气道以外的实质组织。除了支气管相关淋巴组织和支气管周围结缔组织外，在较大的传导气道（第2-6代）中几乎没有可见颗粒蓄积的证据。相反，从每个途径产生的终末和呼吸性细支气管显示不同程度的壁增厚和重塑。壁有显着增厚含有中度到重度的碳质和矿物粉尘。管壁增厚与胶原蛋白和间质炎性细胞（包括含尘巨噬细胞）增加相关。与第二代和第三代呼吸性细支气管相比，第一代呼吸性细支气管的这些变化显著更大。这些结果表明，肺中的碳质和矿物粉尘的积累是显着影响肺解剖与最大的保留在肺腺泡中心。此外，在暴露于环境颗粒物的人体中，该过渡区有显著的重塑。
We examined the relationship between intrapulmonary particle distribution of carbonaceous and mineral dusts and remodeling of the airways along anatomically distinct airway paths in the lungs of Hispanic males from the central valley of California. Lung autopsy specimens from the Fresno County Coroner's Office were prepared by intratracheal instillation of 2% glutaraldehyde at 30 cm H(2)O pressure. Two distinct airway paths into the apico-posterior and apico-anterior portions of the left upper lung lobe were followed. Tissue samples for histologic analysis were generally taken from the intrapulmonary second, fourth, sixth, and ninth airway generations. Parenchymal tissues beyond the 12th airway generation of each airway path were also analyzed. There was little evidence of visible particle accumulation in the larger conducting airways (generations 2-6), except in bronchial-associated lymphoid tissues and within peribronchial connective tissue. In contrast, terminal and respiratory bronchioles arising from each pathway revealed varying degrees of wall thickening and remodeling. Walls with marked thickening contained moderate to heavy amounts of carbonaceous and mineral dusts. Wall thickening was associated with increases in collagen and interstitial inflammatory cells, including dust-laden macrophages. These changes were significantly greater in first-generation respiratory bronchioles compared to second- and third-generation respiratory bronchioles. These findings suggest that accumulation of carbonaceous and mineral dust in the lungs is significantly affected by lung anatomy with the greatest retention in centers of lung acini. Furthermore, there is significant remodeling of this transitional zone in humans exposed to ambient particulate matter.