Bioreduction of chromium (VI) by Bacillus sp isolated from soils of iron mineral area
Bioreduction of chromium (VI) by Bacillus sp isolated from soils of iron mineral area
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从铁矿区土壤中分离出的芽孢杆菌对铬 (VI) 的生物还原
DOI：
10.1016/j.ejsobi.2009.06.009
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发表时间：
2009-09-01
影响因子：
4.2
通讯作者：
Li, Xiaohua
中科院分区：
文献类型：
--
作者：
Cheng, Guojun;Li, Xiaohua
铬在工业中的大量使用造成了环境污染。耐铬细菌能够将有毒的Cr（VI）还原为毒性较小的Cr（III）。8株能在含Cr（VI）500 mg/L的LB培养基上生长。从铁矿区土壤样品中分离到10株放线菌。经16 S rRNA基因序列测定，鉴定为芽孢杆菌属。系统进化树分析表明，分离物可分为两个类群。细菌分离物可以抵抗其他重金属，并在不同水平上还原Cr（VI）。选取一株能耐受2500 mg/L Cr（VI）的菌株（MIDS 05），在10 mg/L Cr（VI）浓度下24 h内几乎100%还原Cr（VI），研究了pH、温度和时间等环境因素对Cr（VI）还原和生长的影响。Cr（VI）对MDS 05细胞生长有一定的影响，当Cr（VI）浓度为100 mg/L时，影响更为明显。MDS 05在5 ~ 50 mg/L的Cr（VI）浓度范围内对Cr（VI）有较好的还原效果，在37 ℃、pH 8条件下的还原效果最好。(C)2009年Elsevier Masson SAS。版权所有
Extensive use of chromium in industry has caused environmental contamination. Chromium-resistant bacteria are capable of reducing toxic Cr (VI) to less toxic Cr (III). Eight isolates, which can grow on LB agar containing 500 mg/L of Cr (VI). were isolated from soil samples of iron mineral area. The bacterial isolates were identified as Bacillus sp. by the 16S rRNA gene sequences. Phylogenetic tree analysis indicates the isolates can be divided into two groups. The bacterial isolates can be resistant to other heavy metals and reduce Cr (VI) at different levels. One bacterial isolate (MIDS05), which can tolerate 2500 mg/L Cr (VI) and was able to reduce almost 100% of Cr (VI) at the concentration of 10 mg/L in 24 h, was selected to study the effects of some environmental factors such as pH, temperature, and time on Cr (VI) reduction and growth. The cell growth of MDS05 was affected by the presence of Cr (VI), especially at the concentration of 100 mg/L. It reduced more amount of Cr (VI) under a wide range of concentrations from 5 to 50 mg/L, and reduction was optimum at 37 degrees C and pH 8.MDS05 showed great promise for use in Cr(VI)detoxification under a wide range of envirommental conditions. (C) 2009 Elsevier Masson SAS. All rights reserved