Establishment of odontoblastic cells, which indicate odontoblast features both in vivo and in vitro
Establishment of odontoblastic cells, which indicate odontoblast features both in vivo and in vitro
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DOI：
10.1111/jop.12080
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发表时间：
2013-11-01
影响因子：
3.3
通讯作者：
Nagatsuka, Hitoshi
中科院分区：
文献类型：
--
作者：
Tsujigiwa, Hidetsugu;Katase, Naoki;Nagatsuka, Hitoshi
牙齿组织工程学为牙齿再生治疗的发展提供了非常有吸引力的前景，它可以治愈牙齿缺失并恢复患者的生活质量。为了进一步完善这种治疗方法，牙髓细胞的分离和培养是一个关键因素。本文报道了一种稳定的GFP转基因大鼠牙髓细胞系TGC（Tooth Matrix-forming，GFP Rat-derived Cell）的建立。TGC在体内外均表现出成牙本质细胞的特征。在体外，TGC暴露于成骨培养基显示胶原纤维合成与基质囊泡和矿化，并在细胞培养皿上形成片状基质。同时观察到ALP活性增加，钙化和牙本质形成相关基因转录水平升高。在体内，将TGC与-TCP颗粒一起移植到SCID小鼠体内，形成了衬有成牙本质细胞的牙本质样和牙髓样结构。值得注意的是，即使在多达80代之后，TGC仍保留其形态特征和分化能力。据我们所知，这是第一个报告的牙髓来源的细胞具有这种稳定的成牙本质细胞的特性。TGC是一种非常有用的牙髓细胞研究模型。
Tooth tissue engineering offers very attractive perspectives for elaboration of regenerative treatments, which enables to cure tooth loss and restore quality of life of the patients. To elaborate such treatment, isolation and culture of dental pulp cell must be achieved as a key element. In this article, we report the establishment of a stable cell line from GFP transgenic rat dental pulp, named TGC (Tooth Matrix-forming, GFP Rat-derived Cell). TGCs have exhibited odontoblastic feature both in vitro and in vivo. In vitro, TGC exposed to osteogenic medium demonstrated collagen fiber synthesis with matrix vesicle and mineralization and formed a sheet-like substrate on the cell culture dish. Increased ALP activity and elevated transcription level of various genes involved in calcification and dentin formation were also observed. In vivo, transplanted TGC in SCID mice with -TCP particles formed dentin-like and pulp-like structure with lining odontoblast. Notably, even after up to 80 passages, TGCs retain their morphological features and differentiation ability. To our knowledge, this is the first report of a dental pulp-derived cell with such stable odontoblastic characteristics. TGC could be a very useful model for further study on dental pulp cell.