Genealogy of the "Green Revolution" gene in rice
Genealogy of the "Green Revolution" gene in rice
复制标题
DOI：
10.1266/ggs.80.351
复制
发表时间：
2005-10-01
影响因子：
1.1
通讯作者：
Sano, Y
中科院分区：
文献类型：
--
作者：
Nagano, H;Onishi, K;Sano, Y
在水稻的“绿色革命”中，利用半矮秆基因（sd 1或OsGA 20 ox 2）培育了高产品种（HYVs）。利用PCR技术对8个野生稻和2个栽培稻的256份材料进行了DGWG型和JKK型两种突变等位基因的检测。DGWG等位基因在一个地方品种（Oryza sativa）和两份野生稻（O. rufipogon），均来自中国，分布范围有限。对OsGA20ox2基因的系统学研究表明，OsGA20ox2基因的62个序列与O. sativa和O. rufipogon包括20个不同的单倍型，表明该物种复合体含有来自两个不同谱系的OsGA20ox2基因。沉默位点的核苷酸差异（pi和theta（w））在粳稻中极低，表明存在遗传瓶颈。单倍型网络显示DGWG和JKK等位基因来源于不同的家系。DGWG携带者（W1944）在该基因座的周围区域具有独特的多态性，表明DGWG等位基因已被保留在野生祖先中，而不是DGWG等位基因已从HYVs渗入到W1944中。虽然半矮秆植物是一个弱的竞争对手在饱和的领域，杂交实验表明，DGWG变异可能已被保存为一个隐藏的变异的遗传背景下的野生稻，没有表现出矮小。
During the "Green Revolution" of rice, high-yielding varieties (HYVs) were developed using a semi-dwarf gene (sd1 or OsGA20ox2). The presence or absence of the two mutant alleles (DGWG type in Dee-geo-woo-gen and JKK type in Jikkoku) were surveyed by PCR using 256 accessions of eight wild and two cultivate rice species. The DGWG allele was detected in a landrace (Oryza sativa) and two accessions of wild rice (O. rufipogon), all of which are from China, showing their limited distribution. Genealogical studies of the OsGA20ox2 gene showed that the 62 sequences of O. sativa and O. rufipogon included 20 distinct haplotypes, indicating that the species complex contained OsGA20ox2 genes from two different lineages. The silent site nucleotide diversities (pi and theta(w)) were extremely low in Japonica rice, suggesting a genetic bottleneck. The haplotype network showed that the DGWG and JKK alleles were derived in different lineages. The DGWG carrier (W1944) had unique polymorphisms in the surrounding region of the locus, suggesting that the DGWG allele has been preserved in the wild progenitor, rather than that the DGWG allele has been introgressed from HYVs to W1944. Although a semi-dwarfing plant is a weak competitor under saturated fields, the crossing experiment revealed that the DGWG variant might have been preserved as a hidden variation in the genetic background of wild rice, without expressing a short-stature.