Intestinal epithelial differentiation: new insights from chimeric and transgenic mice.
Intestinal epithelial differentiation: new insights from chimeric and transgenic mice.
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DOI：
10.1083/jcb.108.4.1187
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发表时间：
1989-04
期刊：
影响因子：
--
通讯作者：
Gordon JI
中科院分区：
文献类型：
--
作者：
Gordon JI
哺乳动物肠上皮细胞经历了连续和快速的更新，是研究细胞分化的独特模型系统。永久分化导致其四种主要细胞类型的产生，每种细胞类型具有独特的表型。增殖和细胞分化的过程在拓扑学上组织得非常好。在该上皮内的几个空间维度中建立并维持基因表达的启动子。最近涉及小鼠聚集嵌合体和转基因小鼠的实验提供了关于肠干细胞起源的见解，细胞分化过程中遵循的迁移途径以及产生肠道基因表达复杂地理差异的分子机制。这个小审查的目的是强调这些强大的方法检查有关肠道上皮生物学的问题的有用性。
T HE mammalian intestinal epithelium represents a unique model system for studying cellular differentiation since it undergoes continuous and rapid renewal. Perpetual differentiation results in the generation of its four principal cell types, each with a unique phenotype. The processes of proliferation and cellular differentiation are topologically very well organized. Gradients in gene expression are established and maintained in several spatial dimensions within this epithelium. Recent experiments involving mouse aggregation chimeras and transgenic mice have provided insights about the origins of intestinal stem cells, the migration pathways followed during cellular differentiation as well as the molecular mechanisms which produce complex geographic differences in gut gene expression. The purpose of this mini-review is to emphasize the usefulness of these powerful methods for examining questions related to enteric epithelial biology.