Mixed-function amine oxidase of the rat hepatocyte nuclear envelope. Demonstration and effects of phenobarbital and 3-methylcholanthrene.
Mixed-function amine oxidase of the rat hepatocyte nuclear envelope. Demonstration and effects of phenobarbital and 3-methylcholanthrene.
复制标题
大鼠肝细胞核膜的混合功能胺氧化酶。
DOI：
10.1016/0006-2952(82)90238-6
复制
发表时间：
1982
影响因子：
5.8
通讯作者：
Kasper,CB
中科院分区：
文献类型：
--
作者：
Sum,CY;Kasper,CB
Mixed-function amine oxidase (EC 1.14.13.8) has been demonstrated in highly purified rat hepatocyte nuclear envelope. The enzyme was present in the nuclear envelope at a level 20 percent of that observed in microsomes. Induction studies indicated that nuclear envelope amine oxidase as well as its microsomal counterpart were refractory to the effects of phenobarbital and 3-methylcholanthrene. Phenobarbital administration increased the specific activity of the microsomalN,N-dimethylanilineN-demethylase and benzo[a]pyrene hydroxylase by 600 and 190 percent, respectively, but decreased the specific activity of the nuclear enzymes by 30–50 percent. In contrast, 3-methylcholanthrene increased the specific activity of benzo[a]pyrene hydroxylase in nuclear envelope and microsomes by 42- and 11-fold, respectively. The hydrocarbon also increased the microsomal and nuclearN,N-dimethylanilineN-demethylase by 40 and 60 percent, respectively, but the specific activity of microsomal and nuclear aniline 4-hydroxylase was decreased by 50 percent. Demonstration of amine oxidase in rat hepatocyte nuclear envelope implicates this enzyme in the toxicity and carcinogenicity of certain drugs and chemicals.
