CLONE-FORMING ABILITY AND DIFFERENTIATION POTENTIAL OF MIGRATORY NEURAL CREST CELLS
CLONE-FORMING ABILITY AND DIFFERENTIATION POTENTIAL OF MIGRATORY NEURAL CREST CELLS
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DOI：
10.1073/pnas.85.14.5325
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发表时间：
1988-07-01
影响因子：
11.1
通讯作者：
LEDOUARIN, NM
中科院分区：
文献类型：
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作者：
BAROFFIO, A;DUPIN, E;LEDOUARIN, NM
脊椎动物胚胎的神经嵴产生多种分化的细胞类型，包括外周神经节的神经元和非神经元细胞（感觉细胞和自主细胞）、色素细胞和中胚层衍生物。从鹌鹑胚胎中提取中脑和后脑周围水平的神经嵴细胞，在3T3饲养层上进行单细胞培养，评价其发育潜力。这些条件被证明对鹌鹑神经嵴细胞的存活、增殖和分化特别有利。在培养7-10天后，对含有1 ~ 20万个细胞的243个克隆进行了分析。表型分析为细胞的存在提供了极具多样性的发育潜能的证据。观察到少数固定的神经元祖细胞和一些多能细胞，能够分化为几种类型的神经元细胞、非神经元细胞、黑素细胞和许多具有中等发育能力的细胞。这些克隆实验揭示了迁移神经嵴细胞在分化能力和增殖潜力方面的显著异质性。
The neural crest of vertebrate embryos gives rise to a variety of differentiated cell types, including neuronal and non-neuronal cells of peripheral ganglia (sensory and autonomic), pigment cells, and mesectodermal derivatives. Neural crest cells were taken from quail embryos at the level encompassing mesencephalon and metencephalon and the developmental potentials were evaluated by culturing them as single cells on 3T3 feeder layers. Such conditions proved to be particularly favorable for survival, proliferation, and differentiation of quail neural crest cells. Two hundred and forty-three clones that contained from 1 to > 20,000 cells were analyzed after 7-10 days of culture. Phenotype analysis provided evidence for the existence of cells with extremely diverse developmental potencies. A few committed neuron progenitors were observed as well as some pleuripotent cells, able to differentiate into several types of neurons, non-neuronal cells, and melanocytes, and many cells with intermediate developmental potencies. These cloning experiments revealed that striking heterogeneity of migrating neural crest cells in terms of their capacity for differentiation and their potential for proliferation.