Measurement of calcium channel inactivation is dependent upon the test pulse potential.
Measurement of calcium channel inactivation is dependent upon the test pulse potential.
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钙通道失活的测量取决于测试脉冲电位。
DOI：
10.1016/s0006-3495(99)77460-6
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Byerly,L
中科院分区：
文献类型：
--
作者：
Gera,S;Byerly,L
我们开发了两种方法来测量淋巴动脉瘤中的钙通道失活-一种方法基于传统的双脉冲方案，在中等大的去极化脉冲期间使用电流，另一种方法在非常强的激活脉冲后使用尾电流。这两种方法都避免了质子电流的污染，不受钙电流衰减的影响。两种方法测量的失活幅度不同；这种差异的产生是因为失活测量本身取决于用于监控钙离子通道电导的测试脉冲电压。我们讨论了两种可以产生这种失活测量的测试脉冲相关性的模型--双通道模型和双开态模型。第一个模型通过假设存在两种类型的钙离子通道来解释这一点，不同的测试脉冲激活了不同比例的钙通道。第二个模型只假设一种钙通道类型，有两个闭合和开放状态；在这个模型中，测试脉冲依赖于测试脉冲在两个闭合状态下对通道的不同激活。钙通道失活测量的测试脉冲依赖性可能是一个被先前研究忽视的普遍现象。
We have developed two methods to measure Ca2+channel inactivation inLymnaeaneurons—one method, based upon the conventional double-pulse protocol, uses currents during a moderately large depolarizing pulse, and the other uses tail currents after a very strong activating pulse. Both methods avoid contamination by proton currents and are unaffected by rundown of Ca2+current. The magnitude of inactivation measured differs for the two methods; this difference arises because the measurement of inactivation is inherently dependent upon the test pulse voltage used to monitor the Ca2+channel conductance. We discuss two models that can generate such test pulse dependence of inactivation measurements—a two-channel model and a two-open-state model. The first model accounts for this by assuming the existence of two types of Ca2+channels, different proportions of which are activated by the different test pulses. The second model assumes only one Ca2+channel type, with two closed and open states; in this model, the test pulse dependence is due to the differential activation of channels in the two closed states by the test pulses. Test pulse dependence of inactivation measurements of Ca2+channels may be a general phenomenon that has been overlooked in previous studies.
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DOI：
--
发表时间：
1996
期刊：
Journal of Physiology
影响因子：
--
作者：
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通讯作者：
R. Penner
影响因子：
64.8
作者：
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通讯作者：
BEAN, BP
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DOI：
--
发表时间：
1995
期刊：
影响因子：
--
作者：
E. Neher
通讯作者：
E. Neher
影响因子：
64.8
作者：
SCULPTOREANU, A;SCHEUER, T;CATTERALL, WA
通讯作者：
CATTERALL, WA
影响因子：
16.2
作者：
OLCESE, R;QIN, N;BIRNBAUMER, L
通讯作者：
BIRNBAUMER, L