Polydatin modulates inflammation by decreasing NF-κB activation and oxidative stress by increasing Gli1, Ptch1, SOD1 expression and ameliorates blood-brain barrier permeability for its neuroprotective effect in pMCAO rat brain
Polydatin modulates inflammation by decreasing NF-κB activation and oxidative stress by increasing Gli1, Ptch1, SOD1 expression and ameliorates blood-brain barrier permeability for its neuroprotective effect in pMCAO rat brain
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DOI：
10.1016/j.brainresbull.2011.09.021
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发表时间：
2012-01-04
影响因子：
3.8
通讯作者：
Li, Litao
中科院分区：
文献类型：
--
作者：
Ji, Hui;Zhang, Xiangjian;Li, Litao
炎症和氧化应激在脑缺血发病机制中起重要作用。虎杖苷已被证明通过其抗炎和抗氧化特性而产生多种生物学效应。然而，关于虎杖苷在缺血性中风中的神经保护机制，人们知之甚少。因此，我们研究了虎杖苷潜在的神经保护作用，并探索了其潜在的机制。雄性SD大鼠永久性大脑中动脉闭塞(PMCAO)模型。实验1检测pMCAO后6个时间点胶质瘤相关癌基因同源1(Gli1)、Patch1(Ptch1)和超氧化物歧化酶1(SOD1)的表达。实验2检测虎杖苷对大鼠大脑中动脉阻塞后的神经保护作用。分别于缺血后24 h和72 h测定神经功能、脑含水量和脑梗塞面积。采用免疫组织化学、逆转录聚合酶链式反应(RT-PCR)、Western blotting、活性测定和共聚焦显微镜等方法检测Gli1、ptch1、SOD1和核因子-kappaB(NF-kappa B)的表达。实验3检测虎杖苷对血脑屏障的影响。与假手术组比较，pMCAO后即刻Gli1、ptch1和SOD1表达上调(P
Inflammation and oxidative stress play an important role in cerebral ischemic pathogenesis. Polydatin has been proved to elicit numerous biological effects through its anti-inflammatory and anti-oxidant properties. However, little is known regard to the mechanism of polydatin's neuroprotection in ischemic stroke. We therefore investigated the potential neuroprotective effects of polydatin and explored the underlying mechanisms. Male, Sprague-Dawley rats were subjected to permanent middle cerebral artery occlusion (pMCAO). Experiment 1 was used to evaluate the expression of glioma-associated oncogene homolog1 (Gli1), Patched-1 (Ptch1) and Superoxide dismutase 1 (SOD1) after pMCAO, six time points were included. Experiment 2 was used to detect polydatin's neuroprotection after pMCAO. Neurological deficit, brain water content and infarct size were measured at 24 h and 72 h after pMCAO. Immunohistochemistry, reverse transcription-polymerase chain reaction (RT-PCR), Western Blotting, activity assay and confocal microscope were used to analyse the expression of Gli1, Ptch1, SOD1 and nuclear factor-kappa B (NF-kappa B). Experiment 3 was used to detect polydatin's influence on blood-brain barrier (BBB). Compared with Sham group, the expression of Gli1, Ptch1 and SOD1 were up-regulated shortly after pMCAO (P