Understanding the human salivary metabolome
Understanding the human salivary metabolome
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DOI：
10.1002/nbm.1369
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发表时间：
2009-07-01
影响因子：
2.9
通讯作者：
Slupsky, Carolyn
中科院分区：
文献类型：
--
作者：
Takeda, Ienaka;Stretch, Cynthia;Slupsky, Carolyn
唾液是一种容易获取的生物流体，对整体健康很重要，有助于咀嚼、吞咽和品尝食物，以及调节口腔菌群。作为确定和了解人类唾液代谢组的第一步，我们测量了口腔卫生状况良好的健康人群在各种条件下的唾液代谢物浓度。使用H-1核磁共振波谱，测量了同一受试者的静息(基础)和刺激唾液中的代谢物浓度，并在一组健康的不吸烟男性受试者(n=62)中进行了比较。与刺激唾液相比，非刺激唾液中的几乎所有代谢物都更高。比较男性吸烟者和不吸烟者(n=46)唾液代谢物的分布，发现吸烟者的柠檬酸、乳酸、丙酮酸和蔗糖的浓度高于非吸烟者，甲酸盐的浓度低于非吸烟者(p<；0.05)。性别差异也被调查(n=40)，男性唾液中的乙酸盐、甲酸盐、甘氨酸、乳酸、甲醇、丙酸、丙二醇、丙酮酸、琥珀酸和牛磺酸的浓度显著高于女性(p<；0.05)。这些结果表明，在唾液代谢物中可能观察到男性和女性、受刺激和未受刺激以及吸烟状态的差异。版权所有(C)2009 John Wiley&Sons，Ltd.
Saliva is a readily accessible biofluid that is important for the overall health, aiding in the chewing, swallowing, and tasting of food as well as the regulation mouth flora. As a first step to determining and understanding the human saliva metabolome, we have measured salivary metabolite concentrations under a variety of conditions in a healthy population with reasonably good oral hygiene. Using H-1 NMR spectroscopy, metabolite concentrations were measured in resting (basal) and stimulated saliva from the same subject and compared in a cohort of healthy male non-smoking subjects (n = 62). Almost all metabolites were higher in the unstimulated saliva when compared to the stimulated saliva. Comparison of the salivary metabolite profile of male smokers and non-smokers (n = 46) revealed citrate, lactate, pyruvate, and sucrose to be higher and formate to be lower in concentration in smokers compared with non-smokers (p < 0.05). Gender differences were also investigated (n = 40), and acetate, formate, glycine, lactate, methanol, propionate, propylene glycol, pyruvate, succinate, and taurine were significantly higher in concentration in male saliva compared to female saliva (p < 0.05). These results show that differences between male and female, stimulated and unstimulated, as well as smoking status may be observed in the salivary metabolome. Copyright (C) 2009 John Wiley & Sons, Ltd.