Analysis of the N-terminal positively charged residues of the simian immunodeficiency virus Vif reveals a critical amino acid required for the antagonism of rhesus APOBEC3D, G, and H.
Analysis of the N-terminal positively charged residues of the simian immunodeficiency virus Vif reveals a critical amino acid required for the antagonism of rhesus APOBEC3D, G, and H.
复制标题
对猿猴免疫缺陷病毒 Vif N 端带正电荷残基的分析揭示了拮抗恒河猴 APOBEC3D、G 和 H 所需的关键氨基酸。
DOI：
10.1016/j.virol.2013.10.037
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Stephens,EdwardB
中科院分区：
文献类型：
--
作者：
Schmitt,Kimberly;Katuwal,Miki;Wang,Yaqiong;Li,Cicy;Stephens,EdwardB
先前的研究已经表明，载脂蛋白B mRNA编辑、酶催化、多肽G（APOBEC 3 G; hA 3 G）和F（APOBEC 3 F; hA 3 F）蛋白与位于HIV-1 Vif蛋白N-末端区域的非线性结合位点相互作用。我们分析了SIV Vif N端12个带正电荷的氨基酸的作用。构建了表达这些氨基酸取代中的每一种的猿猴-人免疫缺陷病毒（SHIV）。检查这些病毒在恒河猴APOBEC 3蛋白（rhA 3A-rhA 3 H）存在下的复制、不同A3蛋白掺入病毒体以及在恒河猴PBMC中的复制。与其他研究相似，我们发现K27对rhA 3G活性和rhA 3F是必需的，但对rhA 3A、rhA 3D或rhA 3 H限制SHIVΔ vif并不重要。我们的研究结果确定了SIV Vif的14位精氨酸是rhA 3D、rhA 3G和rhA 3 H限制病毒的关键残基。
Previous studies have shown that apolipoprotein B mRNA editing, enzyme catalytic, polypeptide G (APOBEC3G; hA3G) and F (APOBEC3F; hA3F) proteins interact with a nonlinear binding site located at the N-terminal region of the HIV-1 Vif protein. We have analyzed the role of 12 positively charged amino acids of the N-terminal region of the SIV Vif. Simian-human immunodeficiency viruses (SHIV) were constructed that expressed each of these amino acid substitutions. These viruses were examined for replication in the presence of rhesus macaque APOBEC3 proteins (rhA3A–rhA3H), incorporation of the different A3 proteins into virions, and replication in rhesus macaque PBMC. Similar to other studies, we found that K27 was essential for rhA3G activity and rhA3F but was not important for restriction of SHIVΔvifby rhA3A, rhA3D or rhA3H. Our results identified the arginine at position 14 of the SIV Vif as a critical residue for virus restriction by rhA3D, rhA3G and rhA3H.