Effect of Sophora subprosrate polysaccharide on oxidative stress induced by PCV2 infection in RAW264.7 cells
Effect of Sophora subprosrate polysaccharide on oxidative stress induced by PCV2 infection in RAW264.7 cells
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苦参多糖对PCV2感染RAW264.7细胞氧化应激的影响
DOI：
10.1016/j.ijbiomac.2013.09.026
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发表时间：
2013-11-01
影响因子：
8.2
通讯作者：
Hu, Ting-Jun
中科院分区：
文献类型：
--
作者：
Su, Zi-Jie;Wei, Ying-Yi;Hu, Ting-Jun
在这项研究中，首先在小鼠巨噬细胞系（RAW 264.7细胞）中通过用猪圆环病毒2型（PCV 2）感染细胞来建立氧化应激模型。研究了苦参多糖（SSP）对PCV 2诱导的氧化应激的调节作用。结果表明，PCV 2感染后，细胞内活性氧（ROS）和一氧化氮（NO）的产生、髓过氧化物酶（MPO）活性和诱导型一氧化氮合酶（iNOS）的表达均显著增加。同时，还原型谷胱甘肽与氧化型谷胱甘肽的比值（GSH/GSSG）和羟自由基防护能力大大降低。这些数据表明在RAW 264.7细胞中成功建立了氧化应激模型。与对照组相比，在暴露于氧化应激的细胞中观察到细胞活力显著降低。当PCV 2感染后用浓度为100、200或400 μ g/mL的SSP处理细胞时，观察到GSH/GSSG比率和羟基自由基预防能力增加。我们还观察到感染细胞中ROS和NO的产生、MPO活性和iNOS表达降低。我们的结果表明，PCV 2感染能够诱导RAW264.7细胞的氧化应激，SSP可以减轻PCV 2感染引起的负面影响。(C)2013爱思唯尔有限公司版权所有。
In this study, an oxidative stress model was first developed in a mouse macrophage cell line (RAW264.7 cells) by infecting the cells with porcine circovirus type 2 (PCV2). The regulatory effect of Sophora subprosrate polysaccharide (SSP) on PCV2-induced oxidative stress was investigated. The results showed that after infection with PCV2, reactive oxygen species (ROS) and nitric oxide (NO) production, myeloperoxidase (MPO) activity, and inducible nitric oxide synthase (iNOS) expression were significantly increased. Meanwhile, the ratio of reduced glutathione to oxidized glutathione (GSH/GSSG) and hydroxyl radical prevention capacity were greatly reduced. These data indicate successful creation of an oxidative stress model in RAW264.7 cells. A dramatic decrease in cell viability was observed in the cells exposed to oxidative stress compared to the control. When the cells were treated with SSP in concentrations of 100, 200 or 400 mu g/mL post PCV2 infection, an increase in the GSH/GSSG ratio and hydroxyl radical prevention capacity was observed. We also observed decreased ROS and NO production, MPO activity, and iNOS expression in the infected cells. Our results demonstrated that PCV2 infection was able to induce oxidative stress in RAW264.7 cells and that SSP could reduce the negative effects resulting from the PCV2 infection. (C) 2013 Elsevier B.V. All rights reserved.