Classification of rice (Oryza sativa L. Japonica nipponbare) immunophilins (FKBPs, CYPs) and expression patterns under water stress.
Classification of rice (Oryza sativa L. Japonica nipponbare) immunophilins (FKBPs, CYPs) and expression patterns under water stress.
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DOI：
10.1186/1471-2229-10-253
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发表时间：
2010-11-18
影响因子：
5.3
通讯作者：
Cho HS
中科院分区：
文献类型：
--
作者：
Ahn JC;Kim DW;You YN;Seok MS;Park JM;Hwang H;Kim BG;Luan S;Park HS;Cho HS
FK506结合蛋白（FKBPs）和亲环蛋白（CYPs）是肽基脯氨酸顺/反式异构酶（PPIase）超家族中含量丰富且普遍存在的蛋白质，它们在蛋白质折叠过程中通过伴侣或脯氨酸残基异构化来调节许多代谢。它们统称为亲免疫蛋白（IMM），存在于几乎所有的细胞器官中。特别地，一些imm与环境压力有关。水稻FKBP和CYP蛋白的研究。考虑到与拟南芥和衣藻的同源关系或蛋白质的分子量，对每个IMM进行了鉴定和分类，并给出了适当的名称。在水稻中鉴定到29个FKBP和27个CYP基因；其中，许多基因可以推定为拟南芥imm的同源基因。然而，有些基因是新基因，与拟南芥和衣藻的基因不匹配，有些基因是通过基因重复而相似的。在水稻56个imm中，有相当数量的imm受盐胁迫和/或干燥胁迫的调控。此外，我们还分析了它们在不同组织中对水分胁迫的表达水平，并利用GFP蛋白标记的方法定位了一些亚细胞imm。与拟南芥（23个FKBPs和29个CYPs）和衣藻（23个FKBPs和26个cyn）等其他绿色光合生物一样，水稻的IMM基因数量在迄今报道的生物中最多，这表明其数量与光合作用密切相关。推测的水稻FKBPs和CYPs的分类提供了与研究相对较好的拟南芥和衣藻属进行比较时它们的进化/功能意义的信息。此外，许多因水分胁迫而上调的基因提供了操纵水稻胁迫反应的可能性。
FK506 binding proteins (FKBPs) and cyclophilins (CYPs) are abundant and ubiquitous proteins belonging to the peptidyl-prolyl cis/trans isomerase (PPIase) superfamily, which regulate much of metabolism through a chaperone or an isomerization of proline residues during protein folding. They are collectively referred to as immunophilin (IMM), being present in almost all cellular organs. In particular, a number of IMMs relate to environmental stresses. FKBP and CYP proteins in rice (Oryza sativa cv. Japonica) were identified and classified, and given the appropriate name for each IMM, considering the ortholog-relation with Arabidopsis and Chlamydomonas or molecular weight of the proteins. 29 FKBP and 27 CYP genes can putatively be identified in rice; among them, a number of genes can be putatively classified as orthologs of Arabidopsis IMMs. However, some genes were novel, did not match with those of Arabidopsis and Chlamydomonas, and several genes were paralogs by genetic duplication. Among 56 IMMs in rice, a significant number are regulated by salt and/or desiccation stress. In addition, their expression levels responding to the water-stress have been analyzed in different tissues, and some subcellular IMMs located by means of tagging with GFP protein. Like other green photosynthetic organisms such as Arabidopsis (23 FKBPs and 29 CYPs) and Chlamydomonas (23 FKBs and 26 CYNs), rice has the highest number of IMM genes among organisms reported so far, suggesting that the numbers relate closely to photosynthesis. Classification of the putative FKBPs and CYPs in rice provides the information about their evolutional/functional significance when comparisons are drawn with the relatively well studied genera, Arabidopsis and Chlamydomonas. In addition, many of the genes upregulated by water stress offer the possibility of manipulating the stress responses in rice.
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