Effects of intrauterine growth restriction on lung liquid dynamics and lung development in fetal sheep
Effects of intrauterine growth restriction on lung liquid dynamics and lung development in fetal sheep
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DOI：
10.1067/mob.2001.108858
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发表时间：
2001-01-01
影响因子：
9.8
通讯作者：
Harding, R
中科院分区：
文献类型：
--
作者：
Cock, ML;Albuquerque, CA;Harding, R
目的：本研究的目的是确定胎儿肺液和肺development.Study设计：宫内生长限制胎儿肺液和肺development. Study设计的影响：宫内生长限制诱导7胎羊从120至140天的妊娠期（术语，约147天的妊娠期）脐胎盘栓塞。我们使用了6只对照胎仔。测量胎肺液体的呼吸和生产率，并在死后（妊娠140天）取出肺，分析表面活性蛋白A、B和C的脱氧核糖核酸、蛋白质和信使核糖核酸的浓度。结果：脐胎盘栓塞诱导胎儿低氧血症、低血糖和宫内生长受限。在妊娠140天，肺重量减少了34%，胎儿肺液体生成率（宫内生长受限组为15.9 +/- 1.8 mL/h，对照组为24.8 +/- 3.9 mL/h）和体积宫内生长受限组的体重减少（宫内生长受限组为110.9 +/- 16.3公吨，对照组为178.1 +/- 11.9公吨）。然而，在调整体重后，数值与对照组无差异。肺脱氧核糖核酸和血浆皮质醇浓度升高，宫内生长受限，但表面活性蛋白的信使核糖核酸水平不变。结论：在宫内生长受限，肺液体和肺的生长与体重成正比，表面活性蛋白的表达不受影响。肺脱氧核糖核酸浓度的变化表明肺可能在结构上不成熟。
OBJECTIVE: The aim of this study was to determine the effects of intrauterine growth restriction on fetal lung liquid and lung development.STUDY DESIGN: Intrauterine growth restriction was induced in 7 fetal sheep from 120 to 140 days' gestation (term, approximately 147 days' gestation) by umbilicoplacental embolization. We used 6 control fetuses. Volumes and production rates of fetal lung liquid were measured, and lungs were removed post mortem (140 days' gestation) for analysis of concentrations of deoxyribonucleic acid, protein, and messenger ribonucleic acid for surfactant proteins A, B, and C.RESULTS: Umbilicoplacental embolization induced fetal hypoxemia, hypoglycemia, and intrauterine growth restriction. At 140 days' gestation lung weight was reduced by 34%, and the fetal lung liquid production rate (15.9 +/- 1.8 mL/h for intrauterine growth restriction vs 24.8 +/- 3.9 mL/h for control) and volume (110.9 +/- 16.3 mt for intrauterine growth restriction vs 178.1 +/- 11.9 mt for control) were reduced in the intrauterine growth restriction group. After adjustment for body weight, however, values were not different from those in the control group. Pulmonary deoxyribonucleic acid and plasma cortisol concentrations were elevated by intrauterine growth restriction, but levels of messenger ribonucleic acid for surfactant proteins were unchanged.CONCLUSION: In intrauterine growth restriction, lung liquid and lung growth were proportionate to body weight, and surfactant protein expression was unaffected. Alterations in lung deoxyribonucleic acid concentrations suggest that the lungs may be structurally immature.