Relationship between the AdeABC efflux system gene content, netilmicin susceptibility and multidrug resistance in a genotypically diverse collection of Acinetobacter baumannii strains
Relationship between the AdeABC efflux system gene content, netilmicin susceptibility and multidrug resistance in a genotypically diverse collection of Acinetobacter baumannii strains
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DOI：
10.1093/jac/dkm231
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发表时间：
2007-09-01
影响因子：
5.2
通讯作者：
Dijkshoorn, Lenie
中科院分区：
文献类型：
--
作者：
Nemec, Alexandr;Maixnerova, Martina;Dijkshoorn, Lenie
目的：了解鲍曼不动杆菌AdeABC外排系统基因的存在及其与耐药的关系。方法：采用聚合酶链式反应(PCR)技术，对116株具有不同基因表型和地理来源的鲍曼不动杆菌进行AdeABC外排系统结构基因(ADEA、ADEB、ADEC)和调控基因(ADER、ADE)的检测，对11种抗菌药物的纸片扩散法检测，奈替米星的MIC检测，以及编码奈替米星修饰酶的aacC2和aA4基因的检测。结果：95株ADEA、ADEB、ADER和ADES基因阳性其中10例为1~3个基因阳性，11例全部为阴性。在49个带有一个或多个其他基因的菌株中发现了ADEC基因。41株菌株最多对一种药物耐药，75株菌株对两种或两种以上药物耐药。奈替米星MIC几乎呈双峰分布，峰值分别为0.5-1和8 mg/L；奈替米星MIC为-gt；=mg/L的6株菌株均为aacC2或aacA4，奈替米星MIC=4 mg/L的61株均为ADABRS阳性且对两种或两种以上药物耐药。
Objectives: To assess the occurrence of the genes of the AdeABC efflux system and their association with antimicrobial resistance in Acinetobacter baumannii.Methods: A set of 116 strains selected for their diversity both in genotypic properties and geographic origin was investigated for the presence of the structural (adeA, adeB and adeC) and regulatory (adeR and adeS) genes of the AdeABC system by PCR, for resistance to 11 antimicrobials by disc diffusion, for MIC of netilmicin and for the presence of aacC2 and aacA4, encoding netilmicin-modifying enzymes.Results: Ninety-five strains were positive for adeA, adeB, adeR and adeS, 10 were positive for 1 to 3 of these genes and 11 were negative for all genes. The adeC gene was found in 49 strains with one or more of the other genes. Forty-one strains were resistant to a maximum of one agent and 75 strains to two or more agents. Netilmicin MICs showed an almost bimodal distribution with respective peaks of 0.5-1 and 8 mg/L; aacC2 or aacA4 was found in six strains with netilmicin MIC of >= 64 mg/L. All 61 strains with netilmicin MICs >= 4mg/L were both adeABRS-positive and resistant to two or more agents, whereas netilmicin MICs