Monodispersed silk fibroin microdroplets for protein stabilization
Monodispersed silk fibroin microdroplets for protein stabilization
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用于稳定蛋白质的单分散丝素蛋白微滴
DOI：
10.1063/1.5026759
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发表时间：
2018-04-23
影响因子：
4
通讯作者：
Fan, Yubo
中科院分区：
文献类型：
--
作者：
Liu, Qiang;Jiang, Nan;Fan, Yubo
乳状液中球状蛋白液滴的稳定性较低，极大地限制了其在药物包封、长期储存和药物控制释放方面的应用。在这里，利用微流控流动聚焦装置来合成辣根过氧化物酶（HRP）负载的丝素蛋白微滴。通过丝素蛋白溶液和95wt. %的向日葵油和5wt. % span 80分别作为分散相和连续相。在这项研究中，油包水丝素蛋白微滴均匀地产生通过利用离散和周期性的微滴的破碎和调节流速。结果表明，包埋在微滴中的HRP的稳定性比未包埋的HRP提高了25%。因此，微流控流动聚焦是一种很有前途的技术，以形成单分散的微滴，并最大限度地提高蛋白质液滴的稳定性。出版社：AIP Publishing
Low stability of globular protein droplets in emulsion significantly limits their applications in drug encapsulation, long-term storage, and controlled drug release. Here, a microfluidic flow-focusing device was utilized to synthesize horseradish peroxidase (HRP)-loaded silk fibroin microdroplets. The two immiscible streams of microfluidic flow-focusing were regenerated by silk fibroin solution and a mixture of 95 wt. % sunflower oil and 5 wt. % span 80 as the dispersed and continuous phases, respectively. In this study, the water-in-oil silk fibroin microdroplets were homogeneously produced by leveraging the discrete and periodic breakup of microdroplets and regulating the flow rates. Moreover, the result showed that the stability of encapsulated HRP in microdroplets was 25% higher than that of HRP after 6 weeks incubation. Thus, the microfluidic flow-focusing is a promising technique to form monodisperse microdroplets and maximize the stability of protein droplets. Published by AIP Publishing.