Incidence of neuronal coupling in supraoptic nuclei of virgin and lactating rats: estimation by neurobiotin and lucifer yellow.
Incidence of neuronal coupling in supraoptic nuclei of virgin and lactating rats: estimation by neurobiotin and lucifer yellow.
复制标题
处女和哺乳大鼠视上核神经元耦合的发生率：通过神经生物素和荧光黄进行估计。
DOI：
10.1016/0006-8993(94)90207-0
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发表时间：
1994
期刊：
影响因子：
2.9
通讯作者：
Yang,QZ
中科院分区：
文献类型：
--
作者：
Hatton,GI;Yang,QZ
神经元之间的染料偶联已被证明反映了电渗性偶联。最近在视网膜上的研究表明，当用神经生物素(NB)转移来估计偶联的发生率时，比用荧光黄(LY)来估计时更高。以前的几项研究表明，哺乳期大鼠视上核(SON)神经元之间LY偶联的发生率是处女大鼠的2-4倍。我们比较了将LY和Nb同时注射到处女鼠或哺乳期大鼠脑片中同一细胞后SON神经元之间的偶联发生率。正如以前的研究所看到的那样，与处女大鼠相比，在哺乳期每次注射LY偶联神经元的数量是未成年大鼠的4倍。在这两种情况下，每次注射的NB偶联神经元的数量是观察到的LY的4倍；讨论了可能的机制。单个充满NB的神经元被连接到分布在SON大片区域的多达10个其他细胞。这些结果证实了先前关于哺乳期儿子比处女儿子更多耦合的发现，并表明神经元间耦合的发生率和空间范围都更大，因此在功能上可能比迄今所认识的更重要。
Dye coupling among neurons has been shown to reflect electrotonic coupling. Recent work in retina has revealed that the incidence of coupling is greater when estimated by neurobiotin (NB) transfer than by Lucifer yellow (LY). Several previous studies have shown that the incidence of LY coupling among supraoptic nucleus (SON) neurons of lactating rats is 2- to 4-fold higher than is observed in virgin females. We compared the incidence of coupling among SON neurons following simultaneous injections of LY and NB into the same cells in slices from virgin or lactating rats. As seen in previous studies, there were 4-fold more LY-coupled neurons per injection in lactating as compared to virgin rats. Under both conditions, the numbers of NB-coupled neurons per injection were 4-fold greater than was observed for LY; possible mechanisms are discussed. Individual NB-filled neurons were coupled to as many as 10 other cells distributed over a large area of the SON. These results confirm previous findings of more coupling in lactating than virgin SONs, and suggest that both the incidence and spatial extent of interneuronal coupling are greater and thus probably more important functionally than has been heretofore appreciated.