In Vitro and In Vivo Evaluation of a Three-Dimensional Porous Multi-Walled Carbon Nanotube Scaffold for Bone Regeneration.
In Vitro and In Vivo Evaluation of a Three-Dimensional Porous Multi-Walled Carbon Nanotube Scaffold for Bone Regeneration.
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DOI：
10.3390/nano7020046
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发表时间：
2017-02-17
期刊：
影响因子：
--
通讯作者：
Saito N
中科院分区：
文献类型：
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作者：
Tanaka M;Sato Y;Zhang M;Haniu H;Okamoto M;Aoki K;Takizawa T;Yoshida K;Sobajima A;Kamanaka T;Kato H;Saito N
碳纳米管（CNTs）由于其独特的物理和生物学特性，在生物和医学领域引起了广泛的关注。在这项研究中，我们研究了三维多孔CNT支架（CNT多孔块; CNTp）的骨再生医学的能力。使用扫描电子显微镜（SEM）的表面观察，在模拟体液（SBF）中浸泡的CNTps的表面上的晶体沉积，并进行蛋白质吸附和控制释放的评价，以评估物理性能。用扫描电镜和荧光显微镜观察细胞增殖情况和细胞形态。将CNTps植入临界大小的小鼠颅骨缺损中，并评价其骨传导能力和重组人BMP-2（rhBMP-2）的体内控制释放。互连多孔HA陶瓷（IP-CHA）用于比较。CNT具有多孔结构，其具有与多壁CNT网络的孔间连接。通过将CNTps浸入SBF中，将含有钙和磷酸盐的晶体沉积在CNTps中和CNT网络的表面上。CNTps比IP-CHAs吸附更明显，释放蛋白更缓慢。前成骨细胞接种到CNTps填充孔与拉伸肌动蛋白丝和丝状伪足。与IP-CHAs相比，CNTps显示出显著更高的细胞增殖，更好的骨传导，以及更多的骨生成与rhBMP-2。在这项研究中，CNTps表现出良好的骨传导能力，细胞附着和增殖能力，以及生长因子保留能力。CNTPs不仅可以作为治疗骨缺损的人工骨，还可以作为组织工程方法再生医学的支架材料。
Carbon nanotubes (CNTs) have attracted a great deal of attention for the biological and medical science fields because of their characteristic physical and biological properties. In this study, we investigated the capacity of the 3D porous CNT scaffold (CNT porous block; CNTp) for bone regenerative medicine. Surface observations using a scanning electron microscope (SEM), crystal depositions on the surface of CNTps immersed in simulated body fluid (SBF), and evaluations of protein adsorption and controlled releasing were conducted to assess physical properties. The cell proliferation and cell morphology were observed using SEM and fluorescent microscopy. CNTps were implanted into critical-size mouse calvarial defects and evaluated for their osteoconductive ability and in vivo controlled release of recombinant human BMP-2 (rhBMP-2). Interconnected porous HA ceramics (IP-CHAs) were used for comparison. CNTps have multiporous structures with interporous connections with networks of multiwalled CNTs. Crystals containing calcium and phosphate were deposited in CNTps and on the surface of the CNT networks by immersing CNTps in SBF. CNTps adsorbed more significantly and released protein more gradually than IP-CHAs. Preosteoblasts seeded onto CNTps filled pores with stretched actin filaments and filopodia. Compared with IP-CHAs, CNTps showed significantly higher cell proliferation, better osteoconduction, and more bone generation with rhBMP-2. In this study, CNTps demonstrated good osteoconductive ability, cell attachment and proliferation capacity, and growth factor retaining ability. CNTps have the potential not only as artificial bones for the treatment of bone defects, but also as scaffolds for regenerative medicine using tissue engineering approaches.