RTG1 AND RTG2 - 2 YEAST GENES REQUIRED FOR A NOVEL PATH OF COMMUNICATION FROM MITOCHONDRIA TO THE NUCLEUS
RTG1 AND RTG2 - 2 YEAST GENES REQUIRED FOR A NOVEL PATH OF COMMUNICATION FROM MITOCHONDRIA TO THE NUCLEUS
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DOI：
10.1016/0092-8674(93)90050-z
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发表时间：
1993-01-15
期刊：
影响因子：
64.5
通讯作者：
BUTOW, RA
中科院分区：
文献类型：
--
作者：
LIAO, XS;BUTOW, RA
一些核基因的表达对线粒体的功能状态敏感，我们称之为逆行调节。在这里，我们表明，逆行调控的酵母ClT 2基因编码过氧化物酶体柠檬酸合酶依赖于一类新的上游激活位点元件（UAS（r））和两个以前身份不明的基因，RTG 1和RTG 2。RTG 1编码一种177个氨基酸的蛋白质，与可能在ClT 2 UAS（r）发挥功能的碱性螺旋-环-螺旋转录因子相似。 RTG 2编码一种功能未知的394个氨基酸的蛋白质。含有RTG 1和RTG 2的无效等位基因的细胞是活的和有呼吸能力的。然而，它们是谷氨酸或天冬氨酸的营养缺陷型，并且不能使用乙酸盐作为唯一的碳源，这表明三羧酸和乙醛酸循环都受到损害。因此，RTG 1和RTG 2是控制线粒体、过氧化物酶体和细胞核之间细胞器间通讯的关键基因。
The expression of some nuclear genes is sensitive to the functional state of mitochondria, a process we term retrograde regulation. Here we show that retrograde regulation of the yeast ClT2 gene encoding peroxisomal citrate synthase depends on a new class of upstream activation site element (UAS(r)) and two previously unidentified genes, RTG1 and RTG2. RTG1 encodes a protein of 177 amino acids with similarity to basic helix-loop-helix transcription factors that likely functions at the ClT2 UAS(r). RTG2 encodes a protein of 394 amino acids of unknown function. Cells containing null alleles of RTG1 and RTG2 are viable and respiratory competent. However, they are auxotrophic for glutamic or aspartic acid and cannot use acetate as a sole carbon source, suggesting that both the tricarboxylic acid and glyoxylate cycles are compromised. Thus, RTG1 and RTG2 are pivotal genes in controlling interorganelle communication between mitochondria, peroxisomes, and the nucleus.