Multiple distinct subtypes of GABAergic neurons in mouse visual cortex identified by triple immunostaining.
Multiple distinct subtypes of GABAergic neurons in mouse visual cortex identified by triple immunostaining.
复制标题
通过三重免疫染色鉴定小鼠视觉皮层中 GABA 能神经元的多种不同亚型。
DOI：
10.3389/neuro.05.003.2007
复制
发表时间：
2007
影响因子：
2.9
通讯作者：
Burkhalter, Andreas
中科院分区：
文献类型：
--
作者：
Gonchar, Yuri;Wang, Quanxin;Burkhalter, Andreas
大多数皮质中间神经元使用GABA（γ-氨基丁酸）作为抑制性神经递质。GABA能神经元在形态、连接、电学和化学上是异质的。在大鼠大脑皮层中，通过小白蛋白（PV）、钙视黄蛋白（CR）和生长抑素（SOM）的表达，鉴定出三组不同的GABA能中间神经元。最近在小鼠大脑皮层的研究揭示了一个不同的组织，其中CR和SOM人口部分重叠。由于CR和SOM神经元来自位于不同胚胎结构中的不同祖细胞，CR + SOM的共表达表明中间神经元的化学分化在有丝分裂后受到调节。在这里，我们已经采取了重要的第一步，通过三重免疫染色小鼠视觉皮层与一组抗体，已被广泛用于分类发展中的中间神经元理解这一过程。我们发现至少有13种不同的GABA能神经元群，包括PV、CR、SOM、CCK、CR + SOM、CR + NPY、CR + VIP、SOM + NPY、SOM + VIP、VIP + ChAT、CCK + NPY、CR + SOM + NPY和CR + SOM + VIP。在出生后发育过程中，PV、CR和SOM抗体的三重免疫染色进一步表明PV从不与CR和SOM共定位。重要的是，SOM和CR + SOM的表达后，不表达SOM的CR细胞的百分比已达到成熟水平，这表明SOM和CR + SOM神经元的化学分化是出生后的事件，这可能是由转录调控。
The majority of cortical interneurons use GABA (gamma amino butyric acid) as inhibitory neurotransmitter. GABAergic neurons are morphologically, connectionally, electrically and chemically heterogeneous. In rat cerebral cortex three distinct groups of GABAergic interneurons have been identified by the expression of parvalbumin (PV), calretinin (CR) and somatostatin (SOM). Recent studies in mouse cerebral cortex have revealed a different organization in which the CR and SOM populations are partially overlapping. Because CR and SOM neurons derive from different progenitors located in different embryonic structures, the coexpression of CR + SOM suggests that the chemical differentiation of interneurons is regulated postmitotically. Here, we have taken an important first step towards understanding this process by triple immunostaining mouse visual cortex with a panel of antibodies, which has been used extensively for classifying developing interneurons. We have found at least 13 distinct groups of GABAergic neurons which include PV, CR, SOM, CCK (cholecystokinin), CR + SOM, CR + NPY (neuropeptide Y), CR + VIP (vasointestinal polypeptide), SOM + NPY, SOM + VIP, VIP + ChAT (choline acetyltransferase), CCK + NPY, CR + SOM + NPY and CR + SOM + VIP expressing cells. Triple immunostaining with PV, CR and SOM antibodies during postnatal development further showed that PV is never colocalized with CR and SOM. Importantly, expression of SOM and CR + SOM developed after the percentage of CR cells that do not express SOM has reached the mature level, suggesting that the chemical differentiation of SOM and CR + SOM neurons is a postnatal event, which may be controlled by transcriptional regulation.