Methods for studying the G2 DNA damage checkpoint in mammalian cells.
Methods for studying the G2 DNA damage checkpoint in mammalian cells.
复制标题
研究哺乳动物细胞中 G2 DNA 损伤检查点的方法。
DOI：
10.1007/978-1-61779-273-1_3
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
O'Connell,MatthewJ
中科院分区：
文献类型：
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作者：
Tapia-Alveal,Claudia;O'Connell,MatthewJ
为了应对复制后 DNA 损伤，细胞激活 G2 DNA 损伤检查点，以确保在损伤修复之前不会尝试有丝分裂。这是对多种 DNA 损伤剂的常见反应，包括电离辐射和许多用于治疗癌症的化疗剂。 G2 DNA 损伤检查点的作用是抑制有丝分裂周期蛋白依赖性激酶，从而使细胞停滞在细胞周期的 G2 期。该检查点的动力学可以通过对细胞有丝分裂标记物进行染色来测定，然后可以通过流式细胞术或显微镜进行量化。
In response to post-replicative DNA damage, cells activate the G2 DNA damage checkpoint to ensure mitosis is not attempted until the damage has been repaired. This is a common response to a variety of DNA damaging agents, including ionizing radiation and many chemotherapeutic agents used in the treatment of cancer. The G2 DNA damage checkpoint acts to inhibit the mitotic cyclin-dependent kinase, and thus cells are arrested in the G2 phase of the cell cycle. The kinetics of this checkpoint can be assayed by staining cells for markers of mitosis, which can then be quantified by flow cytometry or microscopy.