Effects of forskolin on bone in organ culture.
Effects of forskolin on bone in organ culture.
复制标题
毛喉素对器官培养中骨的影响。
DOI：
10.1152/ajpendo.1987.252.1.e44
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Stern,PH
中科院分区：
文献类型：
--
作者：
Krieger,NS;Stern,PH
Forsklin在大多数系统中直接激活腺苷环化酶，它的作用与甲状旁腺激素和降钙素的作用进行了比较，这两种激素都被认为在作用于骨骼时利用cAMP作为第二信使。在体外，Forsklin单独以剂量依赖的方式刺激新生小鼠颅骨和胎鼠四肢骨钙释放。在10(-6)M时，这两个系统的影响都最大。在10~(-4)M浓度范围内，Forsklin可直接刺激甲状旁腺激素与降钙素的相互作用，而降钙素对Forsklin的刺激作用与抑制甲状旁腺激素刺激的骨吸收作用相似。结果表明，Forsklin对骨具有双重作用，在两种骨培养体系中均能模拟甲状旁腺激素和降钙素的反应。观察到的反应取决于福斯科林的使用浓度和药物治疗的时间长短。
The effects of forskolin, which directly activates adenylate cyclase in most systems, have been compared with the actions of parathyroid hormone and calcitonin, both of which have been suggested to utilize cAMP as a second messenger in their actions on bone. Forskolin alone stimulated calcium release from neonatal mouse calvaria and fetal rat limb bones in vitro in a dose-dependent manner. The effect was maximal at 10(-6) M in both systems. At higher concentrations forskolin completely inhibited stimulated bone resorption, although with submaximal concentrations the inhibition was only partially sustained up to 72 h. Forskolin directly stimulated cAMP release from calvaria into the medium at concentrations up to 10(-4) M. Forskolin had no effect on the interaction between parathyroid hormone and calcitonin, while calcitonin inhibited the stimulatory effect of forskolin comparably with its inhibition of parathyroid hormone-stimulated bone resorption. The results indicate that forskolin has dual effects on bone and can mimic responses of both parathyroid hormone and calcitonin in both bone culture systems. The observed response depends on the concentration of forskolin used and the length of treatment with the drug.