Determinations of Carboxyhemoglobin, Methemoglobin, and Cyanide in 70 Blood Samples from Victims Suspected of Carbon Monoxide Poisoning.
Determinations of Carboxyhemoglobin, Methemoglobin, and Cyanide in 70 Blood Samples from Victims Suspected of Carbon Monoxide Poisoning.
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测定 70 份疑似一氧化碳中毒受害者血液样本中的碳氧血红蛋白、高铁血红蛋白和氰化物。
DOI：
10.1248/jhs1956.44.122
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
T. Matsuoka
中科院分区：
文献类型：
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作者：
T. Matsuoka
70 名火灾和汽车尾气受害者的血液中碳氧血红蛋白 (COHb)、高铁血红蛋白 (MetHb) 和氰化物离子 (CN-) 浓度通过我们之前描述的比色法使用气相微循环装置同时测定。 45 分钟内完成一份样品的同时测定。不同一氧化碳中毒源之间血红蛋白衍生物和CN-水平存在差异。如果通过汽车尾气采集的遇难者血样中COHb浓度较低，则应考虑遇难者的死亡原因可能是一氧化碳中毒和缺氧缺氧混合所致。当将 MetHb 浓度用作生命反应的指标时，应仔细确定死亡原因，因为加热火死尸体或即使在 4°C 下保存，血液中的 MetHb 浓度也会发生变化。由于大多数样品中都检测到了CN-，并且部分样品中CN-的致死浓度已确定，因此，当然应该测定从火灾受害者身上采集的血液样品中CN-的浓度。结果表明，COHb、MetHb和CN-浓度的测定对于调查自杀者以及火灾和汽车尾气受害者的死因非常重要，并且本研究中使用的同步方法可用于法医学实践。
Carboxyhemoglobin (COHb), methemoglobin (MetHb), and a cyanide ion (CN-) concentrations in the blood taken from 70 victims by fire and auto exhausts were simultaneously determined by our previously described colorimetries using a gas phase mini-circulation apparatus. The simultaneous determination of one sample was performed within 45 min. There existed a difference of hemoglobin derivatives and CN- levels among the each source of carbon monoxide poisoning. In the case of low COHb concentration in blood samples taken from the victims by auto exhausts, it should be considered that the causes of death of the victims might be owing to the combination of carbon monoxide poisoning and hypoxic hypoxia. When MetHb concentration is used as an indicator for vital reactions, the causes of death should be carefully determined because MetHb concentration in the blood is varied by heating the cadaver died by fire or storing even at 4°C. Since CN- was detected from most of the samples and the lethal level of CN- was determined from the some of the samples, CN- concentration in blood samples taken from the fire victims should be certainly determined. From the results, it was shown that the determination of COHb, MetHb, and CN- concentrations was very important for investigating the causes of death of suicides and victims by fire and auto exhausts, and the simultaneous methods used in this study were available for the practice of forensic science.