Co-cultivation of microalga and xylanolytic bacterium by a continuous two-step strategy to enhance algal lipid production
Co-cultivation of microalga and xylanolytic bacterium by a continuous two-step strategy to enhance algal lipid production
复制标题
通过连续两步策略共培养微藻和木聚糖分解细菌以提高藻类脂质产量
DOI：
10.1016/j.biortech.2021.124953
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发表时间：
2021
影响因子：
11.4
通讯作者：
Jianfei Luo
中科院分区：
文献类型：
--
作者：
Zhangzhang Xie;Weitie Lin;Jianfei Luo
为了提高微藻的脂质产量，将富营养化培养基中的微藻细胞转移到贫营养化培养基中，是目前广泛采用的两步培养策略。然而，运输过程中的收获环节提高了生产成本。为了避免收获步骤，本研究开发了连续两步（CTS）栽培策略。在该策略中，在生物反应器中培养嗜糖小球藻，在嵌入生物反应器的内袋中培养一种木聚糖分解细菌——珍珠胞弧菌；第一步共培养后，取出内袋，开始第二步c的培养。sacchrarophila。在此策略下，脂质产量为825.34 ~ 929.79 mg·L−1，是以葡萄糖为原料的典型两步培养策略的1.7 ~ 1.9倍。在CTS策略中，以木聚糖为原料的共培养促进了微藻的生长，去除内袋产生贫营养化条件，提高了微藻的脂质产量。
To enhance microalgal lipid production, canonical two-step cultivation strategy that by transferring the microalgal cells grown in nutrient-replete medium to nutrient-depleted medium is widely used. However, the harvesting step during the transfer raises the production cost. To avoid the harvesting step, this study developed a continuous two-step (CTS) cultivation strategy. In the strategy,Chlorella sacchrarophilawas grown in bioreactor while a xylanolytic bacteriumCellvibrio pealrivergrown in an inner bag that embedded in the bioreactor; after the first-step co-cultivation, the inner bag is removed which then start the second-step cultivation ofC. sacchrarophila. Based on the strategy, the lipid production was determined as 825.34–929.79 mg·L−1, which were 1.7–1.9 times higher than that of cultivation in canonical two-step strategy using glucose as feedstock. During the CTS strategy, the co-cultivation using xylan as feedstock promotes the microalgal growth and the removal of inner bag produces nutrient-depleted condition for enhancing microalgal lipid production.