The cryopreservation of high concentrated PBMC for dendritic cell (DC)-based cancer immunotherapy
The cryopreservation of high concentrated PBMC for dendritic cell (DC)-based cancer immunotherapy
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DOI：
10.1016/j.cryobiol.2008.12.006
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发表时间：
2009-04-01
期刊：
影响因子：
2.7
通讯作者：
Son, Jeong Hwa
中科院分区：
文献类型：
--
作者：
Heo, Yoon Jeong;Son, Cheol Hun;Son, Jeong Hwa
外周血单核细胞（PBMC）已被接受为使用树突状细胞（DC）或活化淋巴细胞的癌症免疫疗法的独特材料，其正在被开发作为常规疗法如手术、化学疗法和放射治疗的替代或辅助。尽管成功冷冻保存大量PBMC对于免疫治疗是至关重要的，但随后的PBMC冷冻保存对分化为免疫细胞的影响的功能研究尚未得到很好的定义。在本研究中，超过1.0 × 108个细胞/ml的PBMC使用可控速率冷冻机（CRF）冷冻保存长达52周，并储存在液氮罐的气相中。通过比较冻存PBMC与新鲜PBMC来源的未成熟DC（iDC）和成熟DC（mDC）的表型和功能特性，研究了PBMC冻存对分化为DC的影响。结果表明，在相当高的细胞浓度下冷冻保存PBMC不会显著影响PBMC的细胞回收率、活力或表型。冻存PBMC来源的iDC和mDC分化为DC后，具有与新鲜PBMC来源的iDC和mDC相同的典型表型和功能。因此，本研究中描述的改进的PBMC冷冻保存方法可用于基于DC的癌症免疫治疗。(C)2008年爱思唯尔公司All rights reserved.
Peripheral blood mononuclear cells (PBMC) have been accepted as a unique material for cancer immunotherapy using dendritic cells (DC) or activated lymphocytes that are being developed as an alternative or adjuvant to conventional therapies such as surgery, chemotherapy and radiation treatment. Although successful cryopreservation of large numbers of PBMC is critical for the immunotherapy, subsequent functional study of the effects of PBMC cryopreservation on differentiation into immune cells has not been well defined. In this study, over 1.0 x 10(8) cells/ml PBMC were cryopreserved as long as 52 weeks using a controlled-rate freezer (CRF) and stored in a vapor phase of liquid nitrogen tank. The effect of PBMC cryopreservation on differentiation into DC was studied by comparing the phenotypic and functional properties of immature DC (iDC) and mature DC (mDC) derived from cryopreserved PBMC to those from fresh PBMC. The results show that cryopreservation of PBMC at a fairly high cell concentration does not significantly affect cell recovery, viability, or phenotypes of PBMC. After differentiation into DC, iDC and mDC derived from cryopreserved PBMC had their typical phenotypes and function equivalent to those derived from fresh PBMC. Therefore, the improved cryopreservation process of PBMC described in this study is available for DC-based cancer immunotherapy. (C) 2008 Elsevier Inc. All rights reserved.