Structural and distributional variation of mitochondrial rps2 genes in the tribe Triticeae (Poaceae)
Structural and distributional variation of mitochondrial rps2 genes in the tribe Triticeae (Poaceae)
复制标题
DOI：
10.1007/s00122-004-1839-x
复制
发表时间：
2005-04-01
影响因子：
5.4
通讯作者：
Kadowaki, K
中科院分区：
文献类型：
--
作者：
Kubo, N;Salomon, B;Kadowaki, K
与水稻、小麦和玉米的线粒体rps2基因一样，大麦的线粒体rps2基因与低等植物的线粒体rps2基因相比，在3 '区具有延伸的开放阅读框（ORF）。然而，这些谷物的延伸部分是可变的。由于大麦和小麦属于同一族（小麦族），因此了解这两种类型的rps2在进化过程中何时何地产生是很有趣的。为了确定这一点，我们利用线粒体（mt）DNA序列来检查部落小麦族rps2基因的变异。利用3 '端可变区研究了大麦（B）型和小麦（W）型rps2序列在该族19个属中的分布。B型序列在19个属中的10个属中鉴定，而W型序列在所有19个属中均存在。因此，由于存在两个拷贝的基因，所检查的属中有10个具有两种类型的rps2序列。在雀麦族中也存在W型序列，在燕麦族和禾本科中也存在B型序列。基于B型和W型序列构建的系统发育树与基于其他分子数据构建的系统发育树不同。这表明小麦族的线粒体基因组具有独特的进化历史。
The mitochondrial rps2 gene from barley, like that of rice, wheat, and maize, has an extended open reading frame (ORF) at the 3'-region when compared to that from lower plants. However, the extended portions are variable among these cereals. Since barley and wheat belong to the same tribe (Triticeae), it would be interesting to know when and where the two types of rps2 were generated during evolution. To determine this, we utilized the mitochondrial (mt)DNA sequence to examine variations of the rps2 genes in the tribe Triticeae. By means of the variable 3'-region, the distribution of barley (B)-type and wheat (W)-type rps2 sequences was studied in 19 genera of the tribe. The B-type sequence was identified in 10 of the 19 genera, whereas the W-type sequence was present in all 19 genera. Thus, ten of the examined genera have both types of rps2 sequences due to the presence of two copies of the gene. The W-type sequence was also present in the tribe Bromeae and the B-type sequence was also found in Aveneae and Poeae. Phylogenetic trees based on the B-type and W-type sequences were different from those based on other molecular data. This suggests that the mitochondrial genome in Triticeae has a unique evolutionary history.